[Histonet] blurry tissue

[Johnson, Nacaela]

What is the staining intensity like? 


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson <@t> USOncology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Monday, July 25, 2011 12:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] blurry tissue 

I would like some thoughts on how to resolve some "blurry" looking
tissue.  We have had occasional tissue that looks blurry and not crisp
for several weeks now.  It is not all the cases only random tissues.
The tissue is not on the same tissue processor either. We have 2
processors.  The latest cases were a breast, some skins, and a prostate.
I am not certain if this is happening on the tissue processor or in the
stainer.  It has been very humid in our lab so I have started running a
dehumidifier in case there is water in the xylene.  It is so hit and
miss that I am puzzled.   Any suggestions would be greatly appreciated.
Thank you in advance for your thoughts.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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