[Histonet] Controls with patient specimen on same slide

[Mark Tarango]

We do put controls on each slide in a case.  Sometimes it's just one slide
that failed in a run.  A batch control wouldn't tell you which slide failed
if there are no internal controls in the patient tissue.  I personally
wouldn't feel comfortable doing IHC with batch controls.
Mark

On Wed, Jul 20, 2011 at 6:18 AM, Rathborne, Toni <
trathborne <@t> somerset-healthcare.com> wrote:

> All of these responses are great. So here's a follow up question.
> Do you place a control tissue on EACH slide if you have multiple blocks for
> a case, or just on one of the slides?
>
> -----Original Message-----
> From: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
> Sent: Tuesday, July 19, 2011 6:40 PM
> To: histonet <@t> lists.utsouthwestern.edu; Rathborne, Toni
> Subject: Re: [Histonet] Controls with patient specimen on same slide
>
> We do not put our positive control tissue on the test slide; we run batch
> controls.  Many of the unstained slides (breast, GI, and prostate biopsies)
> that we use for IHC testing are cut in our Histology Laboratory as part of a
> part-type slide protocol.  For example, we cut 7 slides, 2 sections on each
> slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if
> needed) #2, 3, 5, and 6 for IHC.  Therefore, it would be very difficult for
> us to place the positive control tissue on the same slide.  In addition, I
> receive a lot of consult cases from other hospitals where they send us
> unstained slides for testing.  Once again, it would be difficult to place
> the positive control tissue on the same slide and it would slow us down in
> terms of starting those slides once they arrive.  However, I think the main
> reason we don't pursue putting the positive control tissue on the same slide
> is the fact that it would consume an enormous amount of control tissue.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs Assistant Director, Anatomic
> Pathology Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
> >>> "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 7/19/2011
> >>> 3:27 PM >>>
>
> Hi,
> I'm interested in knowing how many of you are performing ihc with the
> control tissue and the patient tissue on the same slide. I have seen slides
> available which have designated areas for each tissue to be placed so there
> will not be any confusion. If you're doing it, have you encountered any
> problems? What benefits have you noticed since implementing this process?
> Are your pathologists in favor of this?  If you're not, why not?
> Thanks,
> Toni
>
>
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