[Histonet] Controls with patient specimen on same slide

[Tom McNemar]

Each case gets one positive control per antibody.  If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide

All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides?

-----Original Message-----
From: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
Sent: Tuesday, July 19, 2011 6:40 PM
To: histonet <@t> lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide

We do not put our positive control tissue on the test slide; we run batch controls.  Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol.  For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC.  Therefore, it would be very difficult for us to place the positive control tissue on the same slide.  In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing.  Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive.  However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 7/19/2011
>>> 3:27 PM >>>

Hi,
I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this?  If you're not, why not?
Thanks,
Toni


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