[Histonet] Controls with patient specimen on same slide

[Settembre, Dana]

If I have a cytokeratin AE1/AE3 to run on 5 blocks of the
same patient, I use only 1 positive control slide 
(same slide as patient tissue)
Dana Settembre
University Hospital - UMDNJ
Newark, NJ

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide

All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides?

-----Original Message-----
From: Richard Cartun [mailto:Rcartun <@t> harthosp.org] 
Sent: Tuesday, July 19, 2011 6:40 PM
To: histonet <@t> lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide

We do not put our positive control tissue on the test slide; we run batch controls.  Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol.  For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC.  Therefore, it would be very difficult for us to place the positive control tissue on the same slide.  In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing.  Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive.  However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 7/19/2011 
>>> 3:27 PM >>>

Hi,
I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this?  If you're not, why not?
Thanks,
Toni


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.  The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.  Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.  If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





-------------------------------------------------------------------------
This message was secured by ZixCorp(R).


CONFIDENTIALITY NOTICE
This message and any included attachments are from Somerset Medical Center
and are intended only for the addressee.  The information contained in this
message is confidential and may contain privileged, confidential,
proprietary and/or trade secret information entitled to protection and/or
exemption from disclosure under applicable law.  Unauthorized forwarding,
printing, copying, distribution, or use of such information is strictly
prohibited and may be unlawful.  If you are not the addressee, please
promptly delete this message and notify the sender of the delivery error
by e-mail or you may call Somerset Medical Center's computer Help Desk
at 908-685-2200, ext. 4050.

Be sure to visit Somerset Medical Center's Web site - 
www.somersetmedicalcenter.com - for the most up-to-date news, 
event listings, health information and more.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet