[Histonet] Vasculature in tumors

[Leiker, Merced]

I agree... I've used CD31 to stain tumor vasculature. Some tumor vasculature isn't even lined with endothelium; there's just blood "channels." But most should be lined.

Regards,
Merced

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of JR R
Sent: Friday, July 01, 2011 1:55 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Vasculature in tumors



You are probably looking for capillaries maybe arterioles so there wont be any elastic lamina for the pentachrome stain to detect.

Instead try IHC with an endothelial marker like VE Cadherin (best) or in a pinch, CD31 or PECAM.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology




> Date: Fri, 1 Jul 2011 12:48:33 -0500
> From: sgoebel <@t> mirnarx.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Vasculature in tumors
> 
> Hello all and happy long weekend (yes our boss gave us the rest of the 
> day off at 2!!!)
> 
>  
> 
> Looking to stain vasculature in tumors.  I did trichrome, pentachrome, 
> and PAS (with digestion) and am being told that these are not optimal 
> for what they are looking for.  Any other ideas to stain blood vessels 
> would be awesome!!
> 
>  
> 
> Thanks ya'll!
> 
>  
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> 
> Histotechnologist
> 
> Mirna Therapeutics
> 
> 2150 Woodward Street
> 
> Suite 100
> 
> Austin, Texas  78744
> 
> (512)901-0900 ext. 6912
> 
>  
> 
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