[Histonet] Re: Mercury free Gilson's fluid

[Jorge Tornero]

Hello, Robert, Michael and all those keeping an eye on this list,

Thank you very much for your fast (instant, I'll say, taking into account
our respective timezones) answer to my question.

Best regards,

Jorge Tornero
Instituto Español de Oceanografía-Cádiz
Spain
www.ieo.es

2011/1/28 Robert Richmond <rsrichmond <@t> gmail.com>

> Gilson - whoever he may have been - the name is probably French,
> pronounced zheelSAW - apparently published both mercury and zinc forms
> of the fixative, more than a century ago. My source is the venerable
> Microtomist's Vade-Mecum, 11th ed. 1950, pages 40 and 43.
>
> The mercury formula can be conveniently accessed at
> http://stainsfile.info/StainsFile/prepare/fix/fixatives/gilson.htm
>
> Is there enough nitric acid in the brew to interfere with IHC?
>
> The zinc formula uses
>
> glacial acetic acid 5 mL
> 80% nitric acid 5 mL
> 80% alcohol 100 mL
> distilled water 300 mL
> "dry zinc chloride" 20 grams
>
> Published by Gilson in La Cellule, vi, 1890, page 122.
>
> Petrunkevitch's fixative is closely related, both historically and in
> composition.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>