[Histonet] Re: Processors
Miyamoto, Garret T Mr CIV USA USAMEDCOM
garret.t.miyamoto <@t> us.army.mil
Fri Jan 28 14:05:54 CST 2011
Kathy,
We use both processors and they work well and are happy with them. The only disadvantage I found with the Excelsior was the internal containers that hold the solutions. You cannot see the levels of the solutions. The level indicator on the screen is not always accurate. There is a function that you can use to check the fluid levels but it is time consuming since you have to pump the solution you are checking into the processing chamber.
Garret Miyamoto
Tripler Army Medical Center.
----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
Date: Thursday, January 27, 2011 5:57 pm
Subject: Histonet Digest, Vol 86, Issue 37
To: histonet <@t> lists.utsouthwestern.edu
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. RE: Cytoplasmic staining with Ki67 in frozen sections
> (C.M. van der Loos)
> 2. Re: RE: Rat Skin (Victoria Baker)
> 3. AW: [Histonet] Oil Red O for FS on Muscle (Gudrun Lang)
> 4. Bacterial contamination (Breeden, Sara)
> 5. Re: Bacterial contamination (BSullivan <@t> shorememorial.org)
> 6. proper ventilation (Curt Tague)
> 7. Paraffin (Chiriboga, Luis)
> 8. Re: Paraffin (Grantham, Andrea L - (algranth))
> 9. RE: Paraffin (sgoebel <@t> mirnarx.com)
> 10. GSH meeting - hotel deadline (Shirley A. Powell)
> 11. Re: Oil Red O for FS on Muscle (Catherine Simonson)
> 12. Bacterial Contamination (Breeden, Sara)
> 13. Oil Red O for FS on Muscle (Michelle MacVeigh-Aloni)
> 14. Re: Bacterial contamination (histotech <@t> imagesbyhopper.com)
> 15. Oil Red O for FS on Muscle (Michelle MacVeigh-Aloni)
> 16. Cytology vs. Histology (Beth.Fye <@t> HCAhealthcare.com)
> 17. PROCESSORS (Kathy M. Gorham)
> 18. RE: Bacterial contamination (Tony Henwood)
> 19. Re: PROCESSORS (Rene J Buesa)
> 20. FOXP1 (Debbie Nannenga)
> 21. consultation Bodian-luxol (Mezme Moni)
> 22. Invitation to connect on LinkedIn (Douglas Gregg via LinkedIn)
> 23. RE: PROCESSORS (Langenberg, Stacey)
>
>
> -------------------------------------------------------------------
> ---
>
> Message: 1
> Date: Thu, 27 Jan 2011 19:07:57 +0100
> From: "C.M. van der Loos" <
> Subject: [Histonet] RE: Cytoplasmic staining with Ki67 in frozen
> sections
> To: sonya.martin <@t> soton.ac.uk
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=us-ascii
>
> Dear Sonya,Acetone is an excellent fixative for many antibody to stain frozens, however nuclear antigens like Ki67 after acetone fixation get a bit fuzzy. I would strongly recommend to fix the slides 5 min at room temp in standard 4% buffered formalin, wash with PBS (or TBS) and start your IHC. You will see a much sharper staining result that is more confined to the nucleus.Hope this helps.Cheers, ChrisChris van der Loos, PhD
> Dept. of Pathology
> Academic Medical Center M2-230
> Meibergdreef 9
> NL-1105 AZ Amsterdam
> The Netherlands
>
> Date: Thu, 27 Jan 2011 09:36:29 +0000
> From: "James S." <
> Subject: [Histonet] Cytoplasmic staining with Ki67 in frozen sections
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> histonet <@t> lists.utsouthwestern.edu
> HI All,
>
> I've just been trying out a Ki67 antibody from Abcam (rabbit polyclonal) on some frozen mouse tissues. Staining looked ok in the colon with nuclear localisation in the cells of the basement membrane. However, what I'm really interested in is the spleen and the staining here was a mess! There was some nuclear staining especially in germinal centres and scattered around the white pulp but the red pulp contained clusters of cells with cytoplasmic staining only (I don't know if this is real staining, non-specific staining or an artefact), also there was bright particulate 'staining' all over the red pulp but not the white.
>
> Frozen sections were cut (10um), air dried overnight, fixed in acetone (10mins), blocked with 5% normal goat serum, Ki67 Ab for 2hrs room temp, secondary was goat anti rabbit AlexaFluor 488 (Invitrogen - clean on its own).
>
> I thought this Ki67 staining was going to be easy!!
>
> Any suggestions?
>
> Thanks
> Sonya
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 27 Jan 2011 13:18:07 -0500
> From: Victoria Baker <
> Subject: Re: [Histonet] RE: Rat Skin
> To: "Shaw, Sharon" <
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <
> Message-ID:
> <
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi
>
> What Dolores has said is correct. Zinc formalin is a good fixative for
> skin. Rodent skin has additional difficulties because of the fur and the
> keratin involved with the follicles/hair. The tissue sections should not be
> more than 3 -4 mm in thickness. Also having that large a piece of tissue
> in a cassette (length) can also contribute to some problems - not just with
> fixation but with how it is placed in the cassette for processing, then
> embedding and I'm sure with cutting. My experments with rodent (rat &
> mouse) had all fixation done off of the processor, on a shaker (belly dancer
> platform) for twenty four hours. Tissue was then washed in running tap
> water for thirty minutes and then placed in 70% ethanol for processing. The
> ratio of solution/tissue was 20X. No fixation was done on the processor as
> it would interfere with our other protocols. The actual processing time was
> 10.5 hours. I started with 70%, 80%, 2 - 95%, 2 - abs. etoh, 50/50 -
> abs/xylene, 2 - xylene and 4- changes paraffin. I was using an older Tissue
> tec processor with heat for reagents at 37 degrees C w/ vacuum and
> pressure. Watch your temperatures on the processor because if reagents
> become too hot it will dry out and damage the tissue. Paraffin was set at
> 65 degrees C using paraplast 2 also using vacuum and pressure.
>
> Not knowing what your protocol may be sort of leaves me giving you only
> generalities, if there is anything that I can assist you with further let me
> know.
>
> Good luck.
>
> Vikki
>
>
>
>
> On Thu, Jan 27, 2011 at 12:35 PM, Fischer, Dolores <Dolores_Fischer <@t> baxter.com> wrote:
>
> > The section of skin is too large. It should be no thicker than
> > approximately 3mm. If you need to see the entire piece of skin, you should
> > be able to submit multiple thin sections. A standard 12hr processing
> > schedule works. I am not familiar with zinc formalin, but the standard
> > fixation time for formalin is, minimally 24hrs, therefore I would guess your
> > fixation time is also too short.
> >
> > Dolores
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> > histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon
> > Sent: Thursday, January 27, 2011 10:39 AM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Rat Skin
> >
> > Can anybody share their protocol on processing rat skin I seem to be having
> > a problem with it being under processed. I fix the tissue for 11hrs in zinc
> > formalin and then run a 12 hour processing schedule the skin size is
> > 2.5cmx1cm.
> >
> > Thanks,
> > Sharon
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > The information transmitted is intended only for the person(s)or entity to
> > which it is addressed and may contain confidential and/or legally privileged
> > material. Delivery of this message to any person other than the intended
> > recipient(s) is not intended in any way to waive privilege or
> > confidentiality. Any review, retransmission, dissemination or other use of ,
> > or taking of any action in reliance upon, this information by entities other
> > than the intended recipient is prohibited. If you receive this in error,
> > please contact the sender and delete the material from any computer.
> >
> > For Translation:
> >
> > http://www.baxter.com/email_disclaimer
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 27 Jan 2011 19:27:04 +0100
> From: "Gudrun Lang" <
> Subject: AW: [Histonet] Oil Red O for FS on Muscle
> To: "'Akemi Allison'" <
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Akemi,
> we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To
> prevent the precipitates on the slide, we stain in a plastic coplin jar with
> a screwed tap. The dye-precipitates accumulate on the ground. We take care
> not to mix them up while putting in the slide. So we need no filtering
> before staining. We only stain frozens of lung without fixation.
>
> I don�t know the formula of Oil Red O solution, but perhaps it can be
> handled like the sudan III.
>
> bye
> Gudrun
>
> -----Urspr�ngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Akemi
> Allison
> Gesendet: Mittwoch, 26. J�nner 2011 18:16
> An: histonet
> Betreff: [Histonet] Oil Red O for FS on Muscle
>
> Happy hump day!
>
> Does anyone have a good procedure for Oil Red O for FS on Muscle.
> The procedure the lab I am working with is having a great deal of
> problems with their muscle biopsy panel. I am trouble-shooting some
> of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am
> working with them with pH issues.
>
> The Oil Red O is very problematic. They are mixing before use and
> filtering with 42 Watman paper, but there is a lot of residual
> background on the slides. Also, they were not fixing the sections
> with 37% formaldehyde, even though the procedure calls for it. Could
> you share who you get the stain from also. Your assistance is
> greatly appreciated.
>
> Akemi
>
>
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377 <@t> yahoo.com
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 27 Jan 2011 11:39:07 -0700
> From: "Breeden, Sara" <
> Subject: [Histonet] Bacterial contamination
> To: "Histonet" <
> Cc: "Ragsdale, John" <
> Message-ID:
> <
>
> Content-Type: text/plain; charset="US-ASCII"
>
> My pathologist tells me I have floating bacteria in both special stains
> I did this morning (GMS and Gram); some slides have these floating
> critters and some don't. Because the only common solutions are those
> for processing and deparaffinization and because these bacteria appear
> to be floating above the plane of the tissue - I can't figure out where
> to start looking. My DI water is from a central source and is
> routinely quality-checked, and this is a new building (Sep. 2010) I
> don't want to blame that. Knowing full well that I am probably
> overlooking the obvious, I'm asking for help figuring this out. I need
> a Sputnik Moment. Thanks!
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM 87102
>
> 505-383-9278 (Histology Lab)
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 27 Jan 2011 13:41:42 -0500
> From: BSullivan <@t> shorememorial.org
> Subject: Re: [Histonet] Bacterial contamination
> To: "Breeden, Sara" <
> Cc: Histonet <, "Ragsdale, John"
> <, histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <
>
> Content-Type: text/plain; charset=US-ASCII
>
> One place you need to look is the floatation bath where you cut your
> slides.
>
> Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
> AP Supervisor
> Shore Memorial Hospital
> 609-653-3590
>
>
> Speak only well of people and you need never whisper
>
>
>
> "Breeden, Sara"
> < su.edu> To
> Sent by: "Histonet"
> histonet-bounces@ <
> lists.utsouthwest cc
> ern.edu "Ragsdale, John"
> <
> Subject
> 01/27/2011 01:39 [Histonet] Bacterial contamination
> PM
>
>
>
>
>
>
>
>
>
> My pathologist tells me I have floating bacteria in both special stains
> I did this morning (GMS and Gram); some slides have these floating
> critters and some don't. Because the only common solutions are those
> for processing and deparaffinization and because these bacteria appear
> to be floating above the plane of the tissue - I can't figure out where
> to start looking. My DI water is from a central source and is
> routinely quality-checked, and this is a new building (Sep. 2010) I
> don't want to blame that. Knowing full well that I am probably
> overlooking the obvious, I'm asking for help figuring this out. I need
> a Sputnik Moment. Thanks!
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM 87102
>
> 505-383-9278 (Histology Lab)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 27 Jan 2011 10:56:58 -0800
> From: "Curt Tague" <
> Subject: [Histonet] proper ventilation
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> I want to make some evaluations of my lab ventilation system and perhaps any
> upgrades that might be necessary. Does anyone have any recommendations for
> the Southern California area?
>
>
>
> Thank,
>
> Curt
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 27 Jan 2011 14:06:32 -0500
> From: "Chiriboga, Luis" <
> Subject: [Histonet] Paraffin
> To: "'Histonet <@t> lists.utsouthwestern.edu'"
> <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> HI all
> Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues. I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I would also be interested in other species specific information if you have. You do not have to provide vendor info and feel free to email me off the net. If people are interested, I can compile and resend to the histonet
>
> Thanks
> L
>
>
> Luis Chiriboga
> Experimental Pathology Core Laboratory
> New York University School of Medicine
>
>
>
> <
> <
> <
> ------------------------------------------------------------<
> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<
> =================================
> <
> <
> <
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 27 Jan 2011 11:21:37 -0800
> From: "Grantham, Andrea L - (algranth)" <
> Subject: Re: [Histonet] Paraffin
> To: "Chiriboga, Luis" <
> Cc: "Histonet <@t> lists.utsouthwestern.edu"
> <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> I use Gem Cut Paraffin, Pink Sapphire. I like the way it ribbons and I'm using it in the processor and for embedding. I process/cut animal tissue. It comes in other colors but we are all girls here so we chose pink.
>
> Andi Grantham
>
>
>
> On Jan 27, 2011, at 12:06 PM, Chiriboga, Luis wrote:
>
> > HI all
> > Just wanted to get peoples opinion's on the paraffin you use for processing animal tissues. I am interested in why you use a particular type, pros and cons. This query would apply to mouse and rat but I would also be interested in other species specific information if you have. You do not have to provide vendor info and feel free to email me off the net. If people are interested, I can compile and resend to the histonet
> >
> > Thanks
> > L
> >
> >
> > Luis Chiriboga
> > Experimental Pathology Core Laboratory
> > New York University School of Medicine
> >
> >
> >
> > <
> > <
> > <
> > ------------------------------------------------------------<
> > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<
> > =================================
> > <
> > <
> > <
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 27 Jan 2011 13:23:56 -0600
> From: <
> Subject: RE: [Histonet] Paraffin
> To: <, <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> I have always liked paraplast. It's good because you don't seem to have
> as much tissue separation from the paraffin in the block. It also seems
> to hold up longer on the water bath to give sections a chance to
> "de-wrinkle" themselves without having to pull so much with forceps. It
> also doesn't seem to be as oily as some other paraffins.
> Just my two cents =)
>
> Sarah Goebel, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Chiriboga, Luis
> Sent: Thursday, January 27, 2011 1:07 PM
> To: 'Histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] Paraffin
>
> HI all
> Just wanted to get peoples opinion's on the paraffin you use for
> processing animal tissues. I am interested in why you use a particular
> type, pros and cons. This query would apply to mouse and rat but I
> would also be interested in other species specific information if you
> have. You do not have to provide vendor info and feel free to email me
> off the net. If people are interested, I can compile and resend to the
> histonet
>
> Thanks
> L
>
>
> Luis Chiriboga
> Experimental Pathology Core Laboratory
> New York University School of Medicine
>
>
>
> <
> <
> <
> ------------------------------------------------------------<
> This email message, including any attachments, is for the sole use of
> the intended recipient(s) and may contain information that is
> proprietary, confidential, and exempt from disclosure under applicable
> law. Any unauthorized review, use, disclosure, or distribution is
> prohibited. If you have received this email in error please notify the
> sender by return email and delete the original message. Please note, the
> recipient should check this email and any attachments for the presence
> of viruses. The organization accepts no liability for any damage caused
> by any virus transmitted by this email.<
> =================================
> <
> <
> <
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 10
> Date: Thu, 27 Jan 2011 14:30:42 -0500
> From: "Shirley A. Powell" <
> Subject: [Histonet] GSH meeting - hotel deadline
> To: "histonet <@t> lists.utsouthwestern.edu"
> <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Georgia, Alabama, ALL histotechs,
>
> The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge.
>
> The deadline for making hotel reservations is March 1, 2011 so that gives you a month to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx
>
> Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh<http://www.histosearch.com/gsh> then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance.
>
> Come TRANSFORM yourselves.
>
>
> Shirley Powell
> GSH Secretary
>
>
>
> Shirley A. Powell, HT(ASCP)HTL, QIHC
> Technical Director
> Histology Curricular Support Laboratory
> Mercer University School of Medicine
> 1550 College Street
> Macon, GA 31207
> 478-301-2374 Lab
> 478-301-5489 Fax
>
>
>
> ------------------------------
>
> Message: 11
> Date: Thu, 27 Jan 2011 14:12:53 -0500
> From: Catherine Simonson <
> Subject: Re: [Histonet] Oil Red O for FS on Muscle
> To: gu.lang <@t> gmx.at
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> Content-Type: text/plain; charset=windows-1252
>
> Hello Akemi,
>
> One thing though, how do you "mix" your oil red o? just a stir bar? I
> found that if you don't shake it very vigorously for about 30 seconds before
> filtering you will get that background staining. Remember to shake it
> (shaken, not stirred)
>
> Catherine
>
> On Thu, Jan 27, 2011 at 1:27 PM, Gudrun Lang < wrote:
>
> > Hi Akemi,
> > we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To
> > prevent the precipitates on the slide, we stain in a plastic coplin jar
> > with
> > a screwed tap. The dye-precipitates accumulate on the ground. We take care
> > not to mix them up while putting in the slide. So we need no filtering
> > before staining. We only stain frozens of lung without fixation.
> >
> > I don�t know the formula of Oil Red O solution, but perhaps it can be
> > handled like the sudan III.
> >
> > bye
> > Gudrun
> >
> > -----Urspr�ngliche Nachricht-----
> > Von: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Akemi
> > Allison
> > Gesendet: Mittwoch, 26. J�nner 2011 18:16
> > An: histonet
> > Betreff: [Histonet] Oil Red O for FS on Muscle
> >
> > Happy hump day!
> >
> > Does anyone have a good procedure for Oil Red O for FS on Muscle.
> > The procedure the lab I am working with is having a great deal of
> > problems with their muscle biopsy panel. I am trouble-shooting some
> > of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am
> > working with them with pH issues.
> >
> > The Oil Red O is very problematic. They are mixing before use and
> > filtering with 42 Watman paper, but there is a lot of residual
> > background on the slides. Also, they were not fixing the sections
> > with 37% formaldehyde, even though the procedure calls for it. Could
> > you share who you get the stain from also. Your assistance is
> > greatly appreciated.
> >
> > Akemi
> >
> >
> > Akemi Allison BS, HT (ASCP) HTL
> > Director
> > Phoenix Lab Consulting
> > Tele: 408.335.9994
> > E-Mail: akemiat3377 <@t> yahoo.com
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 12
> Date: Thu, 27 Jan 2011 13:42:06 -0700
> From: "Breeden, Sara" <
> Subject: [Histonet] Bacterial Contamination
> To: "Histonet" <
> Cc: "Ragsdale, John" <
> Message-ID:
> <
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Thanks to everyone that sent in their suggestions. I especially liked
> the one that suggested I inform all my pathologists that special stains
> are unconstitutional. Seriously, though, I'll give all your suggestions
> a shot and see if I can get rid of the little rascals. Thanks again!
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM 87102
>
> 505-383-9278 (Histology Lab)
>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Thu, 27 Jan 2011 13:09:20 -0800
> From: "Michelle MacVeigh-Aloni" <
> Subject: [Histonet] Oil Red O for FS on Muscle
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Akemi,
>
>
>
> Order the stain from Electron Microscopy Sciences - cat # 26503-02. It
> comes as 250 ml solution and is a lot less expensive than some other
> sources. I guess you can probably get it through VWR.
>
>
>
> Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10
> min.
>
> Filter through coarse filter paper. I have even used paper towel with no
> problem. Anything finer will take forever to filter.
>
> Fix the sections with regular 10% NBF for few min. 3-5
>
> Rinse slides in dist. Water few times
>
> Working Oil -red-O for 10 min. I use the plastic containers for shipping
> slides, because the stain will color the plastic and I can easily and
> cheaply replace those, but you can wash them and reuse them multiple times.
>
> 70% alcohol - quick rinse to clear background
>
> Dist. Water - 5 min
>
> Mayers Hematoxylin 5 min
>
> Tap water 10 min
>
> Dist. water rinse or up to 1 min
>
> Mount with Aqua Mount
>
>
>
> Never had any problems. Normal muscle will have very, very tiny droplets. We
> used it on rodent muscle, but it should work the same for human.
>
>
>
> Good luck
>
> Michelle Aloni
>
>
>
> Research Specialist
>
> USC Keck School of Medicine
>
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Thu, 27 Jan 2011 16:22:51 -0500
> From: "histotech <@t> imagesbyhopper.com" <
> Subject: Re: [Histonet] Bacterial contamination
> To: "BSullivan <@t> shorememorial.org" <
> Cc: Histonet <,
> "histonet-bounces <@t> lists.utsouthwestern.edu"
> <, "Ragsdale, John"
> <
> Message-ID: <
> Content-Type: text/plain; charset=us-ascii
>
> Another place to check is the "holding" water that the slides rest in just prior to staining. We run our slides to water on the H&E stainer and then transfer the rack to a "holding" water dish. We bleach this dish nightly, as we have found it contaminated in the past. We don't know what caused the contamination, but we have not it since the bleaching started! ;o)
>
> Good luck!
>
> Michelle
>
>
> On Jan 27, 2011, at 1:41 PM, BSullivan <@t> shorememorial.org wrote:
>
> > One place you need to look is the floatation bath where you cut your
> > slides.
> >
> > Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
> > AP Supervisor
> > Shore Memorial Hospital
> > 609-653-3590
> >
> >
> > Speak only well of people and you need never whisper
> >
> >
> >
> > "Breeden, Sara"
> > <> su.edu> To
> > Sent by: "Histonet"
> > histonet-bounces@ <
> > lists.utsouthwest cc
> > ern.edu "Ragsdale, John"
> > <
> > Subject
> > 01/27/2011 01:39 [Histonet] Bacterial contamination
> > PM
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > My pathologist tells me I have floating bacteria in both special stains
> > I did this morning (GMS and Gram); some slides have these floating
> > critters and some don't. Because the only common solutions are those
> > for processing and deparaffinization and because these bacteria appear
> > to be floating above the plane of the tissue - I can't figure out where
> > to start looking. My DI water is from a central source and is
> > routinely quality-checked, and this is a new building (Sep. 2010) I
> > don't want to blame that. Knowing full well that I am probably
> > overlooking the obvious, I'm asking for help figuring this out. I need
> > a Sputnik Moment. Thanks!
> >
> >
> >
> > Sally Breeden, HT(ASCP)
> >
> > New Mexico Department of Agriculture
> >
> > Veterinary Diagnostic Services
> >
> > 1101 Camino de Salud NE
> >
> > Albuquerque, NM 87102
> >
> > 505-383-9278 (Histology Lab)
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> ------------------------------
>
> Message: 15
> Date: Thu, 27 Jan 2011 13:25:44 -0800
> From: "Michelle MacVeigh-Aloni" <
> Subject: [Histonet] Oil Red O for FS on Muscle
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Few more things:
>
>
>
> 1. The mixed/working solution is good for 1-2 hours only. If you
> filter it for a long time, this might use the good working time and then you
> have trouble, because it just won't work well for you. I just poor the stain
> and the water in a glass cylinder and shake it. That's it.
>
> 2. After coverslipping, do not press on the coverslip to chase air
> bubbles away or this might displace the lipid droplets from their original
> place.
>
> 3. Look at the slides as soon as possible. In few days, you will see
> that the stain is changing into ugly black crystals and the longer you wait
> the larger the crystals = have to repeat it all over again J
>
> 4. To clean the glassware, spray All Purpose Cleaner, use a brush and
> warm water
>
>
>
> Michelle
>
>
>
> ------------------------------
>
> Message: 16
> Date: Thu, 27 Jan 2011 15:29:08 -0600
> From: <
> Subject: [Histonet] Cytology vs. Histology
> To: <
> Message-ID:
> <
>
> Content-Type: text/plain; charset="us-ascii"
>
> I am a Cytotech that now functions as the Pathology Manager over both Histology and Cytology. There is no easy answer and it totally depends on the work environment. At our hospital, the Cytotechs also prep their work, and we do no GYN cytology. So they spend an equal time between bench work and screening. That is not true of many work environments. As far as Histology, it also depends on your work environment. Some Histotechs spend the whole day in front of the Microtome which can also be tough. Histology requires a lot of hand/eye coordination to make a quality slide. I think that is a skill that not everyone can do (well). Both are very good careers, good luck with your decision.
>
> Beth A. Fye, CT (ASCP)
> Pathology Technical Manager
> HCA Richmond Hospital Laboratories
> office: (804)228-6564
> fax: (804)323-8638
> <mailto:Beth.Fye <@t> hcahealthcare.com>
>
>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Thu, 27 Jan 2011 13:41:15 -0800
> From: "Kathy M. Gorham" <
> Subject: [Histonet] PROCESSORS
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> We are looking at purchasing a new processor. I would like any feed
> back negative and positive for the Shandon Excelsior ES vs. the VIP 6.
> Thanks you. You are always so helpful.
>
> Kathy Gorham H.T.
>
>
> GRH National Recognition
> Outstanding Rural Health Organization of 2009 awarded by NRHA
> Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
> Leader in Innovative Excellence 2009 awarded by the OAHHS
> Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center
> Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet
>
> GRH Mission
> We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services.
>
>
> GRH Confidentiality Notice
> This e-mail and any attached documents are for the intended recipient/s only
> and should be protected against viewing by unauthorized persons. The information
> herein may have been disclosed from records whose confidentiality is protected
> by Federal and State Law. Federal regulations prohibit further distribution or
> copying of this information without permission. If you received this e-mail
> transmission in error, please notify the sender immediately to arrange for return
> or destruction of this information.
>
>
>
> ------------------------------
>
> Message: 18
> Date: Thu, 27 Jan 2011 21:47:03 +0000
> From: Tony Henwood <
> Subject: [Histonet] RE: Bacterial contamination
> To: "'Breeden, Sara'" <, Histonet
> <
> Cc: "Ragsdale, John" <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Sally,
>
> It is possible that the bugs are coming from the sectioning water bath. We found that if sections for microbiological staining were cut late in the day, the critters appeared over the slide (not up near the slide label). The controls, which were cut as a batch early morning did not contain the bugs.
>
> The water bath, with extraneous nutrients from the sections, sit around ay 37oC or more and the bugs love it. Try taking a sample to your cytology dept and ask them to do a cytocentrifuge preparation (air-dried, giemsa (or similar) stained) and have a look.
>
> We try and cut the sections for Gram, ZN and Fungi staining first thing in the morning to alleviate the problem.
>
> Only a thought!
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
> Sent: Friday, 28 January 2011 5:39 AM
> To: Histonet
> Cc: Ragsdale, John
> Subject: [Histonet] Bacterial contamination
>
> My pathologist tells me I have floating bacteria in both special stains I did this morning (GMS and Gram); some slides have these floating
> critters and some don't. Because the only common solutions are those
> for processing and deparaffinization and because these bacteria appear to be floating above the plane of the tissue - I can't figure out where
> to start looking. My DI water is from a central source and is
> routinely quality-checked, and this is a new building (Sep. 2010) I don't want to blame that. Knowing full well that I am probably overlooking the obvious, I'm asking for help figuring this out. I need a Sputnik Moment. Thanks!
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM 87102
>
> 505-383-9278 (Histology Lab)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *********************************************************************************
> This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
> *********************************************************************************
>
>
>
> ------------------------------
>
> Message: 19
> Date: Thu, 27 Jan 2011 13:57:08 -0800 (PST)
> From: Rene J Buesa <
> Subject: Re: [Histonet] PROCESSORS
> To: histonet <@t> lists.utsouthwestern.edu, "Kathy M. Gorham"
> <
> Message-ID: <
> Content-Type: text/plain; charset=iso-8859-1
>
> I always recommend ANY VIP over�ANY other. My experience with several makes me do it.
> Ren� J.
>
> --- On Thu, 1/27/11, Kathy M. Gorham < wrote:
>
>
> From: Kathy M. Gorham <
> Subject: [Histonet] PROCESSORS
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, January 27, 2011, 4:41 PM
>
>
> We are looking at purchasing a new processor.� I would like any feed
> back negative and positive for the Shandon Excelsior ES vs. the VIP 6.
> Thanks you.� You are always so helpful.�
>
> Kathy Gorham H.T.
>
>
> GRH National Recognition
> Outstanding Rural Health Organization of 2009 awarded by NRHA
> Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
> Leader in Innovative Excellence 2009 awarded by the OAHHS
> Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center
> Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet
>
> GRH Mission
> We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services.
>
>
> GRH Confidentiality Notice
> This e-mail and any attached documents are for the intended recipient/s only
> and should be protected against viewing by unauthorized persons. The information
> herein may have been disclosed from records whose confidentiality is protected
> by Federal and State Law. Federal regulations prohibit further distribution or
> copying of this information without permission.� If you received this e-mail
> transmission in error, please notify the sender immediately to arrange for return
> or destruction of this information.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 20
> Date: Thu, 27 Jan 2011 15:48:26 -0800
> From: Debbie Nannenga <
> Subject: [Histonet] FOXP1
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> I'm wondering who in IHC land may be using FOXP1? Are you using the polyclonal or the mouse monoclonal (which clone)? Why do you prefer one over the other? Any advvice will be more than welcome.
>
> Thank you.
> Debbie Nannenga, HTL(ASCP), QIHC
> InCyte Pathology
> Spokane Valley, WA 99216
> dnannenga <@t> incytepathology.com
>
>
> ------------------------------
>
> Message: 21
> Date: Thu, 27 Jan 2011 17:29:05 -0800 (PST)
> From: Mezme Moni <
> Subject: [Histonet] consultation Bodian-luxol
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=utf-8
>
> Hello,
> I would like to know if someone has the protocol for doing Bodian-luxol in human
> brain.
>
>
> Thanks
> Monica M.
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Fri, 28 Jan 2011 03:42:56 +0000 (UTC)
> From: Douglas Gregg via LinkedIn <
> Subject: [Histonet] Invitation to connect on LinkedIn
> To: Jackie Smith <
> Message-ID:
> <
> Content-Type: text/plain; charset=UTF-8
>
> LinkedIn
> ------------Douglas Gregg requested to add you as a connection on LinkedIn:
> ------------------------------------------
>
> Jackie,
>
> I'd like to add you to my professional network on LinkedIn.
>
> - Douglas
>
> Accept invitation from Douglas Gregg
> http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1083845339_3/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPnPAPcPkQe3cUc359bQdNbj5ccP1lbPwQdj4Rc3sOcz8LrCBxbOYWrSlI/EML_comm_afe/
>
> View invitation from Douglas Gregg
> http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I1083845339_3/3dvejcPdjgUcPwMckALqnpPbOYWrSlI/svi/
>
> ------------------------------------------
>
> Why might connecting with Douglas Gregg be a good idea?
>
> Have a question? Douglas Gregg's network will probably have an answer:
> You can use LinkedIn Answers to distribute your professional questions to Douglas Gregg and your extended network. You can get high-quality answers from experienced professionals.
>
> http://www.linkedin.com/e/yvpgd1-gjgjs6ps-43/ash/inv19_ayn/
>
>
> --
> (c) 2011, LinkedIn Corporation
>
> ------------------------------
>
> Message: 23
> Date: Thu, 27 Jan 2011 20:53:06 -0700
> From: "Langenberg, Stacey" <
> Subject: RE: [Histonet] PROCESSORS
> To: Rene J Buesa <,
> "histonet <@t> lists.utsouthwestern.edu"
> <, "Kathy M. Gorham" <
> Message-ID:
> <
> Content-Type: text/plain; charset="iso-8859-1"
>
> I demoed the VIP 6 and it is a nice machine. I ended up purchasing the ASP 300 from Leica. We liked it so much we are purchasing a second one this week. My 2 cents worth!
> "People are not an interruption of our business. People are our business."
>
> Stacey Langenberg HT (ASCP) QIHC
> Laboratory Manager
> Histology/IF
> CU Dermatopathology Consultants
> 1999 N. Fitzsimons Pkwy Suite 120
> Aurora, CO 80045
> Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa <@t> yahoo.com]
> Sent: Thursday, January 27, 2011 2:57 PM
> To: histonet <@t> lists.utsouthwestern.edu; Kathy M. Gorham
> Subject: Re: [Histonet] PROCESSORS
>
> I always recommend ANY VIP over ANY other. My experience with several makes me do it.
> Ren� J.
>
> --- On Thu, 1/27/11, Kathy M. Gorham < wrote:
>
>
> From: Kathy M. Gorham <
> Subject: [Histonet] PROCESSORS
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thursday, January 27, 2011, 4:41 PM
>
>
> We are looking at purchasing a new processor. I would like any feed
> back negative and positive for the Shandon Excelsior ES vs. the VIP 6.
> Thanks you. You are always so helpful.
>
> Kathy Gorham H.T.
>
>
> GRH National Recognition
> Outstanding Rural Health Organization of 2009 awarded by NRHA
> Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP
> Leader in Innovative Excellence 2009 awarded by the OAHHS
> Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center
> Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet
>
> GRH Mission
> We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services.
>
>
> GRH Confidentiality Notice
> This e-mail and any attached documents are for the intended recipient/s only
> and should be protected against viewing by unauthorized persons. The information
> herein may have been disclosed from records whose confidentiality is protected
> by Federal and State Law. Federal regulations prohibit further distribution or
> copying of this information without permission. If you received this e-mail
> transmission in error, please notify the sender immediately to arrange for return
> or destruction of this information.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 86, Issue 37
> ****************************************
More information about the Histonet
mailing list