[Histonet] Oil Red O for FS on Muscle

Grantham, Andrea L - (algranth) algranth <@t> email.arizona.edu
Wed Jan 26 12:06:19 CST 2011

I've done many of these stains and I get my ORO stain from Poly Scientific. Actually I use the whole kit and I use Freida's protocol in the second edition. That includes fixing in 37% formaldehyde. I filter the ORO just before use and usually get nice clean slides. If there is a residue it isn't much and wipes off easily. I even re-use the ORO stain once in a while and it still comes out great. My slides are often used for quantifying the amount of lipids so I don't counterstain most of the time.

Andi Grantham

On Jan 26, 2011, at 10:15 AM, Akemi Allison wrote:

> Happy hump day!
> Does anyone have a good procedure for Oil Red O for FS on Muscle.   
> The  procedure the lab I am working with is having a great deal of  
> problems with their muscle biopsy panel.  I am trouble-shooting some  
> of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
> working with them with pH issues.
> The Oil Red O is very problematic.  They are mixing before use and  
> filtering with 42 Watman paper, but there is a lot of residual  
> background on the slides.  Also, they were not fixing the sections  
> with 37% formaldehyde, even though the procedure calls for it.  Could  
> you share who you get the stain from also.  Your assistance is  
> greatly appreciated.
> Akemi
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377 <@t> yahoo.com
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