[Histonet] Oil Red O for FS on Muscle

[Setlak, Lisa]

Hi,
We use Oil Red O - but we don't do it on muscle. Our protocol is we filter the whole bottle before we use it and then we apply it to unfixed slides for about 30 minutes. Rinse in tap water, counterstain with Hematoxylin and coverslip with Aquamount. We use it for cytospins and occasionally on various frozen tissues. Our stain is from American Mastertech.
Hope this helps,
Lisa V.
Children's Memorial Hospital
Chicago, IL 60616
773-868-8949

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Wednesday, January 26, 2011 11:16 AM
To: histonet
Subject: [Histonet] Oil Red O for FS on Muscle

Happy hump day!

Does anyone have a good procedure for Oil Red O for FS on Muscle.   
The  procedure the lab I am working with is having a great deal of  
problems with their muscle biopsy panel.  I am trouble-shooting some  
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
working with them with pH issues.

The Oil Red O is very problematic.  They are mixing before use and  
filtering with 42 Watman paper, but there is a lot of residual  
background on the slides.  Also, they were not fixing the sections  
with 37% formaldehyde, even though the procedure calls for it.  Could  
you share who you get the stain from also.  Your assistance is  
greatly appreciated.

Akemi


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

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