[Histonet] Oil Red O for FS on Muscle

[Liz Chlipala]

Allison

We make up our Oil Red O from scratch same day we use it, let it stand 10 minutes, and then filter with a Millipore Stericup 0.22µm, GP Express Plus Membrane, 250ml Receiver Bottle, catalog number: SCGPU02RE under vacuum.  That seems to help a bit with the background and we are working with a really clear solution. We have not run muscles but aortic root and liver on mouse and rat.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Wednesday, January 26, 2011 10:16 AM
To: histonet
Subject: [Histonet] Oil Red O for FS on Muscle

Happy hump day!

Does anyone have a good procedure for Oil Red O for FS on Muscle.   
The  procedure the lab I am working with is having a great deal of  
problems with their muscle biopsy panel.  I am trouble-shooting some  
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
working with them with pH issues.

The Oil Red O is very problematic.  They are mixing before use and  
filtering with 42 Watman paper, but there is a lot of residual  
background on the slides.  Also, they were not fixing the sections  
with 37% formaldehyde, even though the procedure calls for it.  Could  
you share who you get the stain from also.  Your assistance is  
greatly appreciated.

Akemi


Akemi Allison BS, HT (ASCP) HTL
Director
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

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