[Histonet] Oil Red O for FS on Muscle
liz <@t> premierlab.com
Wed Jan 26 11:23:14 CST 2011
We make up our Oil Red O from scratch same day we use it, let it stand 10 minutes, and then filter with a Millipore Stericup 0.22µm, GP Express Plus Membrane, 250ml Receiver Bottle, catalog number: SCGPU02RE under vacuum. That seems to help a bit with the background and we are working with a really clear solution. We have not run muscles but aortic root and liver on mouse and rat.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Wednesday, January 26, 2011 10:16 AM
Subject: [Histonet] Oil Red O for FS on Muscle
Happy hump day!
Does anyone have a good procedure for Oil Red O for FS on Muscle.
The procedure the lab I am working with is having a great deal of
problems with their muscle biopsy panel. I am trouble-shooting some
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am
working with them with pH issues.
The Oil Red O is very problematic. They are mixing before use and
filtering with 42 Watman paper, but there is a lot of residual
background on the slides. Also, they were not fixing the sections
with 37% formaldehyde, even though the procedure calls for it. Could
you share who you get the stain from also. Your assistance is
Akemi Allison BS, HT (ASCP) HTL
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
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