[Histonet] Oil Red O for FS on Muscle
akemiat3377 <@t> yahoo.com
Wed Jan 26 11:15:52 CST 2011
Happy hump day!
Does anyone have a good procedure for Oil Red O for FS on Muscle.
The procedure the lab I am working with is having a great deal of
problems with their muscle biopsy panel. I am trouble-shooting some
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am
working with them with pH issues.
The Oil Red O is very problematic. They are mixing before use and
filtering with 42 Watman paper, but there is a lot of residual
background on the slides. Also, they were not fixing the sections
with 37% formaldehyde, even though the procedure calls for it. Could
you share who you get the stain from also. Your assistance is
Akemi Allison BS, HT (ASCP) HTL
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
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