[Histonet] Oil Red O for FS on Muscle

Akemi Allison akemiat3377 <@t> yahoo.com
Wed Jan 26 11:15:52 CST 2011

Happy hump day!

Does anyone have a good procedure for Oil Red O for FS on Muscle.   
The  procedure the lab I am working with is having a great deal of  
problems with their muscle biopsy panel.  I am trouble-shooting some  
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am  
working with them with pH issues.

The Oil Red O is very problematic.  They are mixing before use and  
filtering with 42 Watman paper, but there is a lot of residual  
background on the slides.  Also, they were not fixing the sections  
with 37% formaldehyde, even though the procedure calls for it.  Could  
you share who you get the stain from also.  Your assistance is  
greatly appreciated.


Akemi Allison BS, HT (ASCP) HTL
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

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