[Histonet] g ratio

[Geoff McAuliffe]

If you want to get the results of your study published in a reputable 
journal you should use the best available method, ie. epoxy sections.

Geoff

On 1/25/2011 5:16 AM, Bataillon Michel wrote:
> Hello,
> We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware.
> Do you think that we can perform this work with thin paraffin slides - 1.5µm (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks.
> Thank you,
> Michel
>
> Michel BATAILLON
> Histology
> Evreux, France
>
> *************************************************************
>
> Ce message et toutes les pièces jointes sont confidentiels et établis à l'attention exclusive de ses destinataires.
> Si vous recevez ce message par erreur, merci de le détruire et d'en avertir immédiatement l'expéditeur.
> Toute utilisation de ce message non conforme à sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite.
> Internet ne permettant pas de garantir l'intégrité de ce message, le CIT décline toute responsabilité au titre de ce message s'il a été altéré, déformé ou falsifié.
>
> Protégeons ensemble l'environnement, n'imprimons ce mail (et ses pièces jointes) que si cela est nécessaire.
>
>
> This transmission and any attachments are confidential and intended solely for the use of the addressee(s).
> If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer.
> In this case, any unauthorized use, copying or distribution is strictly prohibited.
> Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed.
> CIT shall not be liable for this E-mail if corrupted, changed or falsified.
>
> Please consider the environment before printing this E-mail.
>
> *************************************************************
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************