[Histonet] RE: g ratio

[Houston, Ronald]

Michel,

Most studies involving morphometric studies of peripheral nerves involve "thick" plastic sections stained with toluidine blue. In my opinion, you are unlikely to get consistent and acceptable staining results on paraffin sections for the simple reason that the intense blue staining on thick EM sections is more from the osmium tetroxide than the toluidine blue per se.




Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com


700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.NationwideChildrens.org>

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bataillon Michel
Sent: Tuesday, January 25, 2011 5:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] g ratio



Hello,

We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware.

Do you think that we can perform this work with thin paraffin slides - 1.5µm (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks.

Thank you,

Michel



Michel BATAILLON

Histology

Evreux, France



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