[Histonet] g ratio

Bataillon Michel Michel.Bataillon <@t> citox.com
Tue Jan 25 04:16:06 CST 2011

We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware.
Do you think that we can perform this work with thin paraffin slides - 1.5µm (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks.
Thank you,

Evreux, France


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