[Histonet] necropsy on freezed animal samples

[Geoff McAuliffe]

If the tissue is not fixed soon after death the microscopic morphology 
will be terrible, no matter whether the animal is refrigerated or 
frozen. The person suggesting refrigeration/freezing is (obviously) not 
familiar with histological technique.
As someone else on the list suggested, better planning is needed for the 
harvesting of tissues for microscopy.

Geoff

On 1/21/2011 5:05 AM, krishna_adhikari <@t> mail.com wrote:
>
> Dear Group Members,
> I have a strange query, One person suggested, if the animals for necropsy are more and one can not handle the load of necropsy  on the same day.
> Then simply after sacrificing the animals refrigerate the dead animals to reduce the autolysis process and then proceed with the actual necropsy procedures.
>   is it possible to to the necropsy procedures on the freezed animals samples for further histopathology evaluation?
> expert comments are suggested.
> Regards
> krishna
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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