[Histonet] necropsy on freezed animal samples

[Adam .]

I recently had an animal from a previously undescribed mouse model die
suddenly during the weekend. Since I couldn't send it off to necropsy, I
stashed it in the refrigerator until Monday morning. According to the person
who read the histology "Post-mortem degeneration limited evaluation of most
tissues."

So I would agree from experience that this is not a good idea.

Adam

On Fri, Jan 21, 2011 at 10:21 AM, Geoff McAuliffe <mcauliff <@t> umdnj.edu>wrote:

> If the tissue is not fixed soon after death the microscopic morphology will
> be terrible, no matter whether the animal is refrigerated or frozen. The
> person suggesting refrigeration/freezing is (obviously) not familiar with
> histological technique.
> As someone else on the list suggested, better planning is needed for the
> harvesting of tissues for microscopy.
>
> Geoff
>
>
> On 1/21/2011 5:05 AM, krishna_adhikari <@t> mail.com wrote:
>
>>
>> Dear Group Members,
>> I have a strange query, One person suggested, if the animals for necropsy
>> are more and one can not handle the load of necropsy  on the same day.
>> Then simply after sacrificing the animals refrigerate the dead animals to
>> reduce the autolysis process and then proceed with the actual necropsy
>> procedures.
>>  is it possible to to the necropsy procedures on the freezed animals
>> samples for further histopathology evaluation?
>> expert comments are suggested.
>> Regards
>> krishna
>>
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>>
>>
>
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