[Histonet] necropsy on freezed animal samples

[Fischer, Dolores]

Kirshna,

I wouldn't consider this to be a good practice.  I would expect that if the experimental study is important, thought and planning would have dictated that, at time of necropsy, enough resources would be available to properly cover the necropsy procedure.  If an animal unexpectantly dies on study it is sometimes necessary to refrigerate it overnight if there is no personnel available to perform the necropsy (weekends or PM's).  By refrigerating the animals and not getting the tissues samples in fixative ASAP you are compromising the tissue morphology.  Your last sentence talks about freezed animals.  Freezing would create freeze artifact, making the situation worse.  

Dolores

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of krishna_adhikari <@t> mail.com
Sent: Friday, January 21, 2011 4:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] necropsy on freezed animal samples



Dear Group Members,
I have a strange query, One person suggested, if the animals for necropsy are more and one can not handle the load of necropsy  on the same day. 
Then simply after sacrificing the animals refrigerate the dead animals to reduce the autolysis process and then proceed with the actual necropsy procedures.
 is it possible to to the necropsy procedures on the freezed animals samples for further histopathology evaluation?
expert comments are suggested.
Regards
krishna
 
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