[Histonet] IHC Equipment

Fri Feb 25 09:39:15 CST 2011

Sheila

It is impossible to always get everything into an e-mail when you recieve a
general info request.  I thought what you said was great.

Have a wonderful weekend.

Vikki

On Fri, Feb 25, 2011 at 10:25 AM, Sheila Fonner <sfonner <@t> labpath.com> wrote:

>  Vikki,
>
>
>
> You are absolutely right!  We do all of our ISH as overnight runs to avoid
> the problem of space constraint with the detection kits, etc.  I agree that
> all of the instruments have their good and bad points, and it really depends
> on the lab and what kind of specimens/volume you have.
>
>
>
> Didn’t mean to offend anyone.  I REALLY was just trying to help and give my
> opinion based on my own personal use.
>
>
>
> Sheila
>
>
>
>
>
> *From:* Victoria Baker [mailto:bakevictoria <@t> gmail.com]
> *Sent:* Friday, February 25, 2011 10:18 AM
> *To:* Greg Dobbin
> *Cc:* Sheila Fonner; histonet <@t> lists.utsouthwestern.edu; Cynthia Pyse
> *Subject:* Re: [Histonet] IHC Equipment
>
>
>
> In regards to the Ventana Ultra - if you are running PIN-4 or ISH
> simultaneously on this instrument, the kits/ab's/probes  they require take
> up a lot of space on the reagent carousel which limits the number of
> antibodies you can put on and as runs can go some where around 6-hours you
> could find yourself limited in what you can run without careful planning.
>
>
>
> I'm a long time user and believer in Ventana, but BondMax and Intellipath
> do have my loyalties as well.  It depends on the needs of your lab
> (volume/work flow, available lab space and antibody library size), the
> staffing you have available and their abilities with IHC/ISH.  I've done
> this before and the only way to really know if an instrument will work in
> your lab is to demo them and connect with other users of the instrument.
>
>
>
> Vikki
>
>
>
>
>
>
>
>
>
>
>
>
> On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin <gvdobbin <@t> ihis.org> wrote:
>
> The Bond does do ISH. In fact it utilizes the same detection kit so you
> have only to buy the probes, not the additional detection kit that could
> (depending on your volume of ISH requests) expire.
> Greg
>
> Greg Dobbin, R.T.
> Chief Technologist, Anatomic Pathology
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE    C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
>
> "I find that the harder I work, the
> more luck I seem to have."
> - Thomas Jefferson
>
>
> >>> "Sheila Fonner" <sfonner <@t> labpath.com> 2/25/2011 10:20 AM >>>
>
> Cindy,
>
> We used to use the Dako stainer, and we still have it as a back-up if
> necessary, but we have recently (in the last year) bought a Ventana Ultra.
> You can put 30 slides at a time on it, but you do not have to batch the
> slides.  It is a continuous feed machine, which means that as soon as a
> slide is done, you can take it off and run something else.  You do not have
> to have all of the slides from a case together side by side.  It is
> bar-code
> driven and will find the slides no matter where you put them.  Each slide
> drawer runs independently of the others.  Ours has been wonderful.  The
> technical assistance is fantastic also.  They will help you to initially
> work up all of your antibodies.  You can use theirs, or you can use third
> party antibodies and place them in a prep-kit.  You can also use RTU or
> concentrates.  It's really up to you.  I would highly recommend it if you
> have a large volume.  We demo'd the Biocare Intellipath also.  I liked the
> machine, but it was really just a step up from the Dako.  Leica has the
> Bond
> instruments, which a lot of people like, but for us, it didn't work because
> we wanted to be able to do ISH.  Also, the Leica limits you to drawers of
> ten slides.  So when you load a drawer, with 1 slide or 10, you can't use
> it
> again until that run is finished.  Hope this helps.  If you have any
> questions, you can contact me.
> Have a great day!
>
> Sheila
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia
> Pyse
> Sent: Wednesday, February 23, 2011 2:37 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC Equipment
>
> Hi Histonetters
>
> I currently use a Dako stainer for my IHC staining. It is a work horse with
> very little problems. It is a older model that we may need to replace in
> the
> near future. What is everyone using out in histoland. I would be perfectly
> willing to purchase another Dako but I want to explore all avenues before
> making a decision. What are the pros and cons of the instruments any of you
> are using. How often is the machine down? What is the capacity? We run the
> Dako twice daily usually to the capacity of 48 slides. I would like to hear
> only from actual user of the instrumentation, no vendors please. This is
> only a fact finding e-mail. Thanks in advance for all your input.
>
> Cindy
>
>
>
> Cindy Pyse, CLT, HT (ASCP)
>
> Laboratory/Histology Supervisor
>
> X-Cell Laboratories
>
> 716-250-9235
>
> e-mail cpyse <@t> x-celllab.com
>
>
>
>
>
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