[Histonet] RRAS

[sgoebel <@t> mirnarx.com]

So...trying to find someone who sells RRAS antibody that isn't Abcam.  I
can't seem to make the Abcam antibody work and I have been asked to try
and get it from a different company.

 

2.  Does anyone have any experience with RRAS?  I have tried dilutions
from 1:500 all the way up to 1:2000.  I keep getting a blush endogenous
stain that I can't seem to get out even after blocking for everything
under the sun.  I am using a normal pH antigen retrieval (DAKO).  Should
I try high pH?  I don't know if that will make the positive staining
come out, I think it will make it worse?

 

Thanks histo-hotties!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912