[Histonet] Drosophila Sectioning

[Grantham, Andrea L - (algranth)]

I have done whiteflies, mosquitoes, honey bees and cockroaches among other insects and insect parts. If you contact me offline maybe I can help you. 

Andi Grantham




On Feb 17, 2011, at 7:46 AM, Gil Sharon wrote:

> Hi All, 
> 
> 
> 
> As a microbial ecologist, working with drosophila, I'm looking for a couple
> of protocols (or papers) for fixation and paraffin-embedding of embryos, 3rd
> instar larvae, pupae and adult D. melanogaster (these sections will later be
> used for fluresecnt-in-situ-hybridization probing for specific bacteria).
> Looking in the literature, I could only find protocols for embryos (or fly
> heads). As I'm really new to histology, I hope someone here will be able to
> help me with this. I have the help of a histo lab here, but they're working
> on mice, so no experience with drosophila (or insects at all). 
> 
> 
> 
> Thanks in advance, 
> 
> Gil.
> 
> 
> 
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