[Histonet] Re: Drosophila sectioning
Damien
dmlaud <@t> gmail.com
Thu Feb 17 09:33:59 CST 2011
Hi Gil,
My histology service specializes in insects. I have a protocol that I can
send you & answer any additional questions you may have. Please feel free to
contact me.
Best,
Damien Laudier
On Thu, Feb 17, 2011 at 9:52 AM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:
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> Today's Topics:
>
> 1. Re: Section position on slides (Victoria Baker)
> 2. Information Please (Jesus Ellin)
> 3. Ventana Rep. (DKBoyd <@t> chs.net)
> 4. RE: Exam Prep (Rochan Moir-Dial)
> 5. Colorado Sate Histo Meeting, April 29-30, 2011 (Matthew Lunetta)
> 6. NY lab permit (Marian Powers)
> 7. neutrophil antibody on rat FFP section (Amy Lee)
> 8. CellSoft vs. Sodium Hydroxide (traczyk7 <@t> aol.com)
> 9. Re: saliva for glycogen hydrolysis (Eric Hoy)
> 10. Re: Novolink polymer kit (amitapandey <@t> torrentpharma.com)
> 11. RE: Histonet Digest, Vol 87, Issue 28 (Ma, Minhui)
> 12. Drosophila Sectioning (Gil Sharon)
> 13. RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP
> section (Patsy Ruegg)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 16 Feb 2011 13:23:02 -0500
> From: Victoria Baker <bakevictoria <@t> gmail.com>
> Subject: Re: [Histonet] Section position on slides
> To: Helen Fedor <hfedor <@t> jhmi.edu>
> Cc: Tanya Ewing-Finchem <tjey <@t> hotmail.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <AANLkTi=GwzNw+s807X5o7LFKO8z3qea1Lcd1wbJsrEBn <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Automated coverslipping instruments are defined by the manufacturer, but
> modifications on some systems can be done. Most clinical labs who utilize
> automation use 24X50mm - as this coverslip will cover most of the slide.
>
> Tissue placement is pretty much determined by laboratory needs. Sheehan &
> Hrapchak had a short segment about tissue placement, but it was in regards
> to the tissue orientation for microscopic assessment requirements. There
> is
> nothing in granite about tissue placement that I know of.
>
> Some tools that I worked with were using the Cytospin large rectangular
> slides to show residents/students/collegues where the tissue placement
> needed to be. Once they were able to get the tissue section in there we
> moved on. While to most of us who work in Histo sort of take putting
> sections on a slide for granted (yes we did work hard to get there)
> getting
> the mechanics of it takes patience, skill and practice. Keep it simple and
> build up from there.
>
> Vikki
>
>
> On Wed, Feb 16, 2011 at 12:27 PM, Helen Fedor <hfedor <@t> jhmi.edu> wrote:
>
> > Since automation is becoming more and more a part of all Histology labs
> the
> > demands of placement of the tissue on the slides varies for different
> > instruments. Stainers, coverslippers and now with slide scanning as well.
> So
> > I do not believe that there is a silver bullet answer.
> >
> > Helen L. Fedor
> >
> > Tissue Microarray Lab, Manager
> > Prostate Spore Lab, Manager
> > Johns Hopkins University
> > 600 N. Wolfe St, | Marburg Room 406
> > Baltimore, MD | 21287-7065
> >
> > 410.614.1660
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> > histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
> Jeanine
> > (CDC/OID/NCEZID)
> > Sent: Wednesday, February 16, 2011 12:21 PM
> > To: Tanya Ewing-Finchem; histonet <@t> lists.utsouthwestern.edu
> > Subject: RE: [Histonet] Section position on slides
> >
> > I always try to center the section equally from sides and top-bottom as
> > possible. This means measure from the bottom of the frosted edge as the
> > "top". The Artisan special stains system has a clip that attaches around
> > the slide to allow reagents to pool onto the sections and incubate. If
> the
> > section is too close to the sides then these areas do not stain
> adequately.
> > Sometimes if the tissue section itself is very large this is
> unavoidable.
> > With automated coverslippers you must also consider placement of tissue
> to
> > allow for proper coverage.
> >
> > If I am cutting multiple unstained slides for subsequent testing I try to
> > orient the tissue the same on each slide to facilitate the reading of
> these
> > slides by the pathologist's
> >
> > Jeanine Bartlett
> > Infectious Diseases Pathology Branch
> > (404) 639-3590
> > jeanine.bartlett <@t> cdc.hhs.gov
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> > histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya
> > Ewing-Finchem
> > Sent: Wednesday, February 16, 2011 12:09 AM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Section position on slides
> >
> >
> > I am trying to put together a training document around microtomy and
> > sectioning and am finding it hard to find information around the
> placement
> > of the actual sections on the slides. These are the objectives I am
> looking
> > to answer. Is this information found in any publications?
> >
> > 1) Tissue / Section Placement: Are there published guidelines /
> > documentation on precisely where you should place tissue sections on a
> 25mm
> > x 75mm glass slide? Perhaps more importantly, where you should NOT
> place
> > tissue (ie. "x" mm from the edge of the glass slide)?
> >
> > 2) Diagnosable Slide Staining Area: With automation becoming more
> widely
> > used in IHC, are there published guidelines / documentation on the usable
> or
> > diagnosable staining area on a 25mm x 75mm glass slide? For instance,
> would
> > you define that as the area under a traditional coverslip? Would this be
> > defined as the entire slide below the label? Or is this some distance
> from
> > all the edges of the slide? With some automated systems, it is near
> > impossible to get edge to edge staining. Is this acceptable?
> >
> >
> >
> > Thanks for any ideas.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 16 Feb 2011 11:38:04 -0700
> From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
> Subject: [Histonet] Information Please
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <29BE166A2CF48D459853F8EC57CD37E8035D68ED <@t> EXCHANGECLUSTER.yumaregional.local
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Wanting to get information on anyone that has already implemented Epic
> and offered the Epic ambulatory piece to there clients. Are you also
> offering this technology with interface to competitive laboratories
> like, Quest, Lab Corp, etc?
>
>
> ______________________________________________________________________
> This message is confidential, intended only for the named
> recipient(s) and may contain information that is privileged
> or exempt from disclosure under applicable law. If you are
> not the intended recipient(s), you are notified that the
> dissemination, distribution, or copying of this message is
> strictly prohibited. If you receive this message in error,
> or are not the named recipient(s), please notify the sender
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> Thank You.
> ______________________________________________________________________
>
> ------------------------------
>
> Message: 3
> Date: Wed, 16 Feb 2011 13:39:22 -0500
> From: DKBoyd <@t> chs.net
> Subject: [Histonet] Ventana Rep.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF9513C84E.D1B1F41F-ON85257839.0053FABD-85257839.00667B1E <@t> chs.net>
> Content-Type: text/plain; charset="US-ASCII"
>
> Can a Ventana Representative for the Southern Virginia region, please give
> me a call at the number below.
> Please only a Ventana rep. I do not have authorization to deal with
> another company at this time.
> Thank you.
>
> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
> Center I
> 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
> 804-765-5582 l dkboyd <@t> chs.net
>
>
>
>
>
> --------------------------------------------------------------------------
> Disclaimer: This electronic message may contain information that is
> Proprietary, Confidential, or legally privileged or protected. It
> is intended only for the use of the individual(s) and entity named
> in the message. If you are not an intended recipient of this
> message, please notify the sender immediately and delete the
> material from your computer. Do not deliver, distribute or copy
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> reliance on the information it contains.
>
> ------------------------------
>
> Message: 4
> Date: Wed, 16 Feb 2011 11:47:51 -0700
> From: "Rochan Moir-Dial" <rochan <@t> premierlab.com>
> Subject: RE: [Histonet] Exam Prep
> To: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>,
> <caymanfleck <@t> gmail.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <EE33BE5C905A3046A7FF8F58A64C8E4B0A9035 <@t> server.PremierLab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> I also used Freida Carson, Histotechnology A Self-Instructional Test,
> 3rd Edition. As well as, the Freida Carson, Companion Study Flash Cards
> that goes along with the 3rd Edition text book. Study the objectives and
> your golden.
>
> Rochan Moir-Dial, HTL(ASCP)
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> Phone: (303) 682-3949
> Fax (303) 682-9060
> rochan <@t> premierlab.com
> www.premierlab.com
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson,
> Nacaela
> Sent: Wednesday, February 16, 2011 9:24 AM
> To: caymanfleck <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Exam Prep
>
> I just used the Freida Carson Histotechnology A Self-Instructional Test.
>
>
>
> Thanks,
>
> Nacaela Johnson, HTL (ASCP)
> Histotechnologist
> KCCC Pathology
> 12000 110th St., Ste. 400
> Overland Park, KS 66210
> Office: 913-234-0576
> Fax: 913-433-7639
> Email: Nacaela.Johnson <@t> USOncology.com
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> caymanfleck <@t> gmail.com
> Sent: Wednesday, February 16, 2011 10:12 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Exam Prep
>
> Hello Histoland,
>
> I'm preparing for the HTL exam after being trained on-the-job and
> working in histo for a few years. I'm using the standard 'Theory and
> practice...' text as my main resource. I'm wondering if anyone has used
> either the ASCP practice tests (a set of 5 are available from ascp for
> about $30) or the NSH Self-assessment Program (which has modules that
> correspond to the exam sections). The NSH program is expensive at about
> $240 but I don't know if it's worth it. Has anyone used either of these
> resources?
>
> Thanks!
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> </pre>The contents of this electronic mail message and any attachments
> are confidential, possibly privileged and intended for the addressee(s)
> only.<br>Only the addressee(s) may read, disseminate, retain or
> otherwise use this message. If received in error, please immediately
> inform the sender and then delete this message without disclosing its
> contents to anyone.</pre>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 16 Feb 2011 12:16:30 -0700
> From: "Matthew Lunetta" <MLunetta <@t> luhcares.org>
> Subject: [Histonet] Colorado Sate Histo Meeting, April 29-30, 2011
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4D5BC01E020000A800056647 <@t> ns.luhcares.org>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi,
>
> All are welcome. It is a great program this year.
>
> Attached is the program for the 2011 CSH meeting, which will be held April
> 29th & 30th at the Embassy Suites hotel in Loveland, CO. Online registration
> and credit card payment is available on the CSH website at
> http://www.coloradohisto.org/2011/meeting.htm
>
> Ciao
> Matt Lunetta
> HT (ASCP)
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 16 Feb 2011 14:18:23 -0500
> From: Marian Powers <mpowers <@t> dpspa.com>
> Subject: [Histonet] NY lab permit
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <AANLkTimZ9UuuxUo=dUSGmsiPnkmrjoLGFAHCOKyZ1e99 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello all,
>
> Anyone out there have experience in applying for a NY state lab permit?
> How
> long did it take? How difficult was it? Could anyone recommend a lab
> consultant on this task?
>
> Thanks in advance,
>
>
>
> --
> *Marian L. Powers, BSOM, HT(ASCP) *
>
> * *
> * *
> * *
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 16 Feb 2011 12:06:41 -0800 (PST)
> From: Amy Lee <amylee779 <@t> yahoo.com>
> Subject: [Histonet] neutrophil antibody on rat FFP section
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <544851.36245.qm <@t> web38005.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello,
> I was asked to perform neutrophil IHC on rat FFP sections. I searched
> around and found most are for mouse tissue. There is one from Serotec mouse
> anti rat but it does not work on paraffin section.
>
> Could anybody recommend a good antibody?
>
> Thanks in advance,
>
> Amy
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 16 Feb 2011 16:31:36 -0500 (EST)
> From: traczyk7 <@t> aol.com
> Subject: [Histonet] CellSoft vs. Sodium Hydroxide
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8CD9C38063E34B6-12F8-6B35 <@t> webmail-d064.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Greetings one & all:
> Has anyone tried "CellSoft" from CellPath in the UK? I received a sample
> today and after reviewing the MSDS that came with it, I'm skeptical about
> the claim in their literature that it "reduces the hazard risk for the
> user". I've got 10% sodium hydroxide on hand for softening nails so if
> anyone has already done a product comparison, I'd love to hear from you.
> Thanks.
> Dorothy
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 16 Feb 2011 15:33:23 -0600
> From: Eric Hoy <Eric.Hoy <@t> UTSouthwestern.edu>
> Subject: [Histonet] Re: saliva for glycogen hydrolysis
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>,
> "histonet-request <@t> lists.utsouthwestern.edu"
> <histonet-request <@t> lists.utsouthwestern.edu>
> Message-ID: <C9819EC3.34070%Eric.Hoy <@t> UTSouthwestern.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> > From: "Ingles Claire " <CIngles <@t> uwhealth.org>
> > Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis
>
> > Wouldn't GUM be a more palatable option? Who knows where some of those
> rubber
> > bands have been!
> > Claire
> >
>
> Back in my early days in the lab, when I was in the US Navy (wooden ships
> and Folin-Wu glucose), I was involved in a project that required us to
> collect fairly large (10-15 mL) saliva samples from Navy recruits who were
> part of a research study. We used a wax gum, which I believe was
> manufactured by Wrigley's gum company. The sticks were the same size and
> shape as gum (back then gum looked like a microscope slide, not all the
> shapes that are currently available) and they were individually wrapped
> like
> gum. They tasted like paraffin, but they induced copious salivation, and
> served our purpose well.
>
> I don't know if this product is still available.
>
> Eric Hoy
>
> ===============================================
> Eric S. Hoy, Ph.D., SI(ASCP)
> Clinical Associate Professor
> Department of Medical Laboratory Sciences
> The University of Texas Southwestern Medical Center
> Dallas, Texas
> Email: Eric.Hoy <@t> UTSouthwestern.edu
> ===============================================
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Thu, 17 Feb 2011 09:43:59 +0530
> From: amitapandey <@t> torrentpharma.com
> Subject: [Histonet] Re: Novolink polymer kit
> To: "MaxVision Biosciences Inc. " <info <@t> maxvisionbio.com>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> OF9E0253B7.324114FD-ON6525783A.0016B216-6525783A.001723E9 <@t> torrentpharma.com
> >
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Thanks Michelle and all histonetters for your suggestions.
>
> After your feed back, i am finalizing the Novolink Polymer-HRP detection
> kit for my work. I am going to use this kit for single labelling of the
> antibody. i will let u know about my result as it comes.
>
> Amita
>
> ------------------------------
>
> Message: 11
> Date: Thu, 17 Feb 2011 09:33:25 -0500
> From: "Ma, Minhui" <mminhui <@t> emory.edu>
> Subject: [Histonet] RE: Histonet Digest, Vol 87, Issue 28
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 43DEC30DB656564EB330190305519FB701ADFE534CAF <@t> EXCHANGE22.Enterprise.emory.net
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear everyone,
>
> I'm wondering where I can order the Picro-Mallory Trichrome Stain kit? I
> couldn't find it online excepted some Italian companies.
> Thanks,
>
> Minhui Ma MD,HTL
> Emory University
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> histonet-request <@t> lists.utsouthwestern.edu
> Sent: Wednesday, February 16, 2011 12:30 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 87, Issue 28
>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. RE: Hema 3/Diff Quik (Wanda.Smith <@t> HCAhealthcare.com)
> 2. HT Position in Oklahoma City (Gaiser, Marcia)
> 3. RE: Re: saliva for glycogen hydrolysis (Ingles Claire )
> 4. Wage question for Wisconsin (Michael Hillmer)
> 5. RE: Wage question for Wisconsin (Ingles Claire )
> 6. Re: Wage question for Wisconsin (Lee & Peggy Wenk)
> 7. Section position on slides (Tanya Ewing-Finchem)
> 8. Novolink Polymer detection kit (amitapandey <@t> torrentpharma.com)
> 9. Re: Section position on slides (Paula Sicurello)
> 10. Na K ATpase immunohistochemistry on rat FFPE tissue
> (amitapandey <@t> torrentpharma.com)
> 11. RE: Wage question for Wisconsin (sgoebel <@t> mirnarx.com)
> 12. RE: Section position on slides (sgoebel <@t> mirnarx.com)
> 13. RE: Re: saliva for glycogen hydrolysis (Gill, Caula A.)
> 14. Re: Wage question for Wisconsin (Victoria Baker)
> 15. Correction, Correction on cutoff date for GSH meeting
> (Shirley A. Powell)
> 16. Pathco double-blade handle (Breeden, Sara)
> 17. RE: Pathco double-blade handle (jstaruk)
> 18. Exam Prep (caymanfleck <@t> gmail.com)
> 19. RE: Exam Prep (Johnson, Nacaela)
> 20. RE: Exam Prep (sgoebel <@t> mirnarx.com)
> 21. Re: Section position on slides (Bill B.)
> 22. RE: Section position on slides
> (Bartlett, Jeanine (CDC/OID/NCEZID))
> 23. RE: Section position on slides (Helen Fedor)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 15 Feb 2011 12:07:09 -0600
> From: <Wanda.Smith <@t> HCAhealthcare.com>
> Subject: [Histonet] RE: Hema 3/Diff Quik
> To: <NKonop <@t> chw.org>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 9E2D36CE2D7CBA4A94D9B22E8328A3BA139EE804DD <@t> NADCWPMSGCMS03.hca.corpad.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Afternoon,
> We use:
> 88333 Touch prep initial site
> 88334 Touch prep addition site
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC 29406
> 843-847-4586
> 843-847-4296 fax
>
> This email and any files transmitted with it may contain PRIVILEGED or
> CONFIDENTIAL information and may be read or used only by the intended
> recipient. If you are not the intended recipient of the email or any of its
> attachments, please be advised that you have received this email in error
> and that any use, dissemination, distribution, forwarding, printing, or
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>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
> Sent: Tuesday, February 15, 2011 12:27 PM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] RE: Hema 3/Diff Quik
>
> I should also include that we are using this diff quik as a rapid stain
> during frozen section in conjunction with a rapid H&E.
>
> Nicole Anne Konop BS, HTL(ASCP)
> Histology Team Lead
> Children's Hospital of Wisconsin
> (414)266-6580 Direct Line
> (414)907-0366 Pager
> (414)266-2524 Histology Department
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
> Sent: Tuesday, February 15, 2011 11:01 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] Hema 3/Diff Quik
>
> I'm looking for information on what CPT codes are being used for the diff
> quik/hema 3 stain? Any feedback is greatly appreciated. Thanks!
>
> Nicole Anne Konop BS, HTL(ASCP)
> Histology Team Lead
> Children's Hospital of Wisconsin
> (414)266-6580 Direct Line
> (414)907-0366 Pager
> (414)266-2524 Histology Department
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 15 Feb 2011 14:25:18 -0600
> From: "Gaiser, Marcia" <Marcia_Gaiser <@t> ssmhc.com>
> Subject: [Histonet] HT Position in Oklahoma City
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 728F817C02110E498D803A7C3B0C6248057D85BD5C <@t> S009-APEXM06.ds.ad.ssmhc.com>
>
> Content-Type: text/plain; charset="Windows-1252"
>
> St. Anthony Hospital currently has an excellent opportunity for an
> experienced Histologic Technician. This position requires Certification as
> an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two
> years of previous histology experience required with IHC and/or grossing
> experience a plus. Outstanding benefits package, including generous paid
> time off. For consideration, please apply online at www.saintsok.com<
> http://www.saintsok.com/<https://mobile.ssmhc.com/owa/UrlBlockedError.aspx
> <https://mobile.ssmhc.com/owa/UrlBlockedError.aspx>>>, Ad # 10762, or
> contact Anna King for additional information at (405) 272-6105.
>
>
>
> Confidentiality Notice: This email message, including any
> attachments, is for the sole use of the intended recipient(s) and
> may contain confidential and privileged information. Any
> unauthorized review, use, disclosure or distribution is prohibited.
> If you are not the intended recipient, please contact the sender by
> reply email and destroy all copies of the original message.
>
> ------------------------------
>
> Message: 3
> Date: Tue, 15 Feb 2011 16:55:24 -0600
> From: "Ingles Claire " <CIngles <@t> uwhealth.org>
> Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> F2F030053F9B7345831BED293A6D57E103A1A6CE <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Wouldn't GUM be a more palatable option? Who knows where some of those
> rubber bands have been!
> Claire
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Robert
> Richmond
> Sent: Mon 2/14/2011 11:09 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: saliva for glycogen hydrolysis
>
>
>
> Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
> spit method (which by the way is still how it's done by the small
> pathology services I work on):
>
> You can produce copious quantities of saliva by the simple expedient
> of chewing on a rubber band for a couple of minutes. This used to be
> the technique used to obtain saliva samples for determination of ABH
> substance secretor status in the blood bank. (I belong to that elite
> 20% of donors who are non-secretors, and Lewis-a positive to prove
> it.)
>
> Bob Richmond
> Samurai pathologist and occasional sialogogue
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 15 Feb 2011 17:02:58 -0600
> From: "Michael Hillmer" <mhillmer <@t> dermwisconsin.com>
> Subject: [Histonet] Wage question for Wisconsin
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 9060F1C2EB3D8A4AAF3B3433C9003230F975A8 <@t> hostexchbe1.hosting.tushausweb.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We are dermatology clinic in Northeast Wisconsin and we are trying to do
> gather accurate wage data. Can anybody offer wages for an HT, HtL and
> lab assistants? Any help would be greatly appreciated.
>
>
>
>
>
>
>
>
>
> Thank you-
>
>
>
> Michael Hillmer PHR
>
> HR Coordinator
>
> Dermatology Associates of Wisconsin
>
> Phone: (920)683-5278
>
> Fax: (920)686-9674
>
> Cell: (920)860-6360
>
>
>
> The materials and information in this e-mail are confidential and may
> contain Protected Health Information covered under the HIPAA Privacy
> Rule. If you are not the intended recipient, be advised that any
> unauthorized use, disclosure, copying, distribution, or action taken in
> reliance on the contents of this information is strictly forbidden by
> law. If you have received this e-mail in error, please notify me by
> reply e-mail and then delete this message. Do not pass any of this
> information to anyone else. Thank you for your cooperation.
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 15 Feb 2011 18:48:09 -0600
> From: "Ingles Claire " <CIngles <@t> uwhealth.org>
> Subject: RE: [Histonet] Wage question for Wisconsin
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> F2F030053F9B7345831BED293A6D57E103A1A6D2 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> The answer to that question may have to wait a week or two. At least here
> at the UW. Our wonderful governor is trying to get rid of the state workers
> unions and collective bargaining. He is trying to push it through (it was
> just introduced this last Friday), and the vote is slated for later this
> week. The techs at the UW hospital have fortunately signed their contract
> that is good until the reopener in 1013 and we are not strictly state
> employees. However, the way things are looking, it is not out of the
> question that he may also try to void our contracts. Our pay MAY go up, but
> we will no longer have much, if anything, for benefits. Don't count on
> sending your kids to the UW colleges if this goes through.
> Sorry, I'm fighting mad right now. I'm not usually big on the unions, but
> this is HUGE for millions of Wisconsinites. GGRRRRR!
> Claire
>
> Were there any other Wisconsin techs at the rally today? Even the Police
> and Firefighters unions were down there!
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Michael
> Hillmer
> Sent: Tue 2/15/2011 5:02 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Wage question for Wisconsin
>
>
>
> We are dermatology clinic in Northeast Wisconsin and we are trying to do
> gather accurate wage data. Can anybody offer wages for an HT, HtL and
> lab assistants? Any help would be greatly appreciated.
>
>
> Thank you-
>
>
>
> Michael Hillmer PHR
>
> HR Coordinator
>
> Dermatology Associates of Wisconsin
>
> Phone: (920)683-5278
>
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 15 Feb 2011 20:27:32 -0500
> From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
> Subject: Re: [Histonet] Wage question for Wisconsin
> To: "Michael Hillmer" <mhillmer <@t> dermwisconsin.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <75338FFC879A4718B6404F650270FE3C <@t> HP2010>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> The following link is to the ASCP "Laboratory Medicine" article from March
> 2009. They do the survey every other year, so there should be another
> survey
> coming out any month now.
>
> http://www.ascp.org/pdf/Membership-Communications/Wage-and-Vacancy-Survey.aspx
>
> Advance for Medical Laboratory Professionals just published their survey on
> Jan. 31, 2011.
>
> http://laboratorian.advanceweb.com/SharedResources/Downloads/2011/013111/MT013111_SalarySurvey.pdf
>
> Realize that even with a state, there can be several dollars per hour
> difference. Let alone the differences between states in a region. I suggest
> taking the information in the surveys, look at the relationship between
> HT/HTL, MLT/MT, and CT, and then apply the same ratio within your own
> institute.
>
> Hope that helps.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Hospital
> Royal Oak, MI 48073
>
> --------------------------------------------------
> From: "Michael Hillmer" <mhillmer <@t> dermwisconsin.com>
> Sent: Tuesday, February 15, 2011 6:02 PM
> To: <histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Wage question for Wisconsin
>
> > We are dermatology clinic in Northeast Wisconsin and we are trying to do
> > gather accurate wage data. Can anybody offer wages for an HT, HtL and
> > lab assistants? Any help would be greatly appreciated.
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > Thank you-
> >
> >
> >
> > Michael Hillmer PHR
> >
> > HR Coordinator
> >
> > Dermatology Associates of Wisconsin
> >
> > Phone: (920)683-5278
> >
> > Fax: (920)686-9674
> >
> > Cell: (920)860-6360
> >
> >
> >
> > The materials and information in this e-mail are confidential and may
> > contain Protected Health Information covered under the HIPAA Privacy
> > Rule. If you are not the intended recipient, be advised that any
> > unauthorized use, disclosure, copying, distribution, or action taken in
> > reliance on the contents of this information is strictly forbidden by
> > law. If you have received this e-mail in error, please notify me by
> > reply e-mail and then delete this message. Do not pass any of this
> > information to anyone else. Thank you for your cooperation.
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 15 Feb 2011 22:09:24 -0700
> From: Tanya Ewing-Finchem <tjey <@t> hotmail.com>
> Subject: [Histonet] Section position on slides
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU157-w10A8B2F000172BF782CF20CDD20 <@t> phx.gbl>
> Content-Type: text/plain; charset="Windows-1252"
>
>
> I am trying to put together a training document around microtomy and
> sectioning and am finding it hard to find information around the placement
> of the actual sections on the slides. These are the objectives I am looking
> to answer. Is this information found in any publications?
>
> 1) Tissue / Section Placement: Are there published guidelines /
> documentation on precisely where you should place tissue sections on a 25mm
> x 75mm glass slide? Perhaps more importantly, where you should NOT place
> tissue (ie. ?x? mm from the edge of the glass slide)?
>
> 2) Diagnosable Slide Staining Area: With automation becoming more widely
> used in IHC, are there published guidelines / documentation on the usable or
> diagnosable staining area on a 25mm x 75mm glass slide? For instance, would
> you define that as the area under a traditional coverslip? Would this be
> defined as the entire slide below the label? Or is this some distance from
> all the edges of the slide? With some automated systems, it is near
> impossible to get edge to edge staining. Is this acceptable?
>
>
> Thanks for any ideas.
>
> ------------------------------
>
> Message: 8
> Date: Wed, 16 Feb 2011 11:39:14 +0530
> From: amitapandey <@t> torrentpharma.com
> Subject: [Histonet] Novolink Polymer detection kit
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> OF0B53C2D6.1196A49A-ON65257839.002127B5-65257839.0021CD79 <@t> torrentpharma.com
> >
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello Histonetters,
>
> This is first time, I am planning to use Novolink polymer detection kit
> for my mouse monoclonal antibody (Millipore Anti Na K ATPase and anti
> vimentin) and rabbit polyclonal antibody (Sc-1506R).
>
> If any body has experience on this polymer kit , please share your
> valuable experience which will help me to set up the method in my lab.
>
> Thanks in advance
> Amita
>
> ------------------------------
>
> Message: 9
> Date: Wed, 16 Feb 2011 07:02:58 -0500
> From: Paula Sicurello <patpxs <@t> gmail.com>
> Subject: Re: [Histonet] Section position on slides
> To: Tanya Ewing-Finchem <tjey <@t> hotmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <AANLkTiksDVmFcb1J0Cgwn5iuUcGF9Cw4MWTVZ0vOfL9Z <@t> mail.gmail.com>
> Content-Type: text/plain; charset=windows-1252
>
> Hi Tanya,
>
> It's always best to train people to place the section in the middle of
> the slide. It depends if one has to put multiple sections on a slide.
> Learning how to do proper placement will help them when they have to
> cut controls for IHC that are often times placed on the top of the
> slide.
>
> Sections placed too close to the very bottom or top sometimes don't
> get stained or coverslipped. Too close to the edges and you can
> suffer wrap around or overlap, causing the section to be difficult for
> the pathologist to read. Boy howdy! They will let you know if they
> don't like the placement.
>
> Therefore, train for centering the section as best as possible.
>
> I hope this helps,
>
> Paula :-)
>
> On Wed, Feb 16, 2011 at 12:09 AM, Tanya Ewing-Finchem <tjey <@t> hotmail.com>
> wrote:
> >
> > I am trying to put together a training document around microtomy and
> sectioning and am finding it hard to find information around the placement
> of the actual sections on the slides. ?These are the objectives I am looking
> to answer. ?Is this information found in any publications?
> >
> > 1) ?Tissue / Section Placement: ?Are there published guidelines /
> documentation on precisely where you should place tissue sections on a 25mm
> x 75mm glass slide? ? Perhaps more importantly, where you should NOT place
> tissue (ie. ?x? mm from the edge of the glass slide)?
> >
> > 2) ?Diagnosable Slide Staining Area: ?With automation becoming more
> widely used in IHC, are there published guidelines / documentation on the
> usable or diagnosable staining area on a 25mm x 75mm glass slide? ?For
> instance, would you define that as the area under a traditional coverslip?
> ?Would this be defined as the entire slide below the label? ?Or is this some
> distance from all the edges of the slide? ?With some automated systems, it
> is near impossible to get edge to edge staining. ?Is this acceptable?
>
> >
> > Thanks for any ideas. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
> _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
> Paula Sicurello, HTL (ASCP)
> Supervisor, Electron Microscope Laboratory
> Duke University Health System
> Rm.#251M, Duke South, Green Zone
> Durham, North Carolina 27710
> P: ?919.684.2091
>
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 16 Feb 2011 17:42:37 +0530
> From: amitapandey <@t> torrentpharma.com
> Subject: [Histonet] Na K ATpase immunohistochemistry on rat FFPE
> tissue
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> OFF2BD856F.83823B44-ON65257839.0042A96B-65257839.00431316 <@t> torrentpharma.com
> >
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Does any body have experienced on Anti Na K ATpase immunohistochemistry on
> FFPE rat kidney tissue?
> I am finding difficult to have any supportive lit regarding this, most of
> them support to frozen sectioning.
>
> Thanks a lot
>
> Amita
>
> ------------------------------
>
> Message: 11
> Date: Wed, 16 Feb 2011 08:56:20 -0600
> From: <sgoebel <@t> mirnarx.com>
> Subject: RE: [Histonet] Wage question for Wisconsin
> To: <CIngles <@t> uwhealth.org>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D957F2A7D21959488C492A2680F9920A1C58F2 <@t> svrexch.asuragen.us>
> Content-Type: text/plain; charset="US-ASCII"
>
> At least you have a union!!
>
> Sarah Goebel, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
> Claire
> Sent: Tuesday, February 15, 2011 6:48 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Wage question for Wisconsin
>
>
> The answer to that question may have to wait a week or two. At least
> here at the UW. Our wonderful governor is trying to get rid of the state
> workers unions and collective bargaining. He is trying to push it
> through (it was just introduced this last Friday), and the vote is
> slated for later this week. The techs at the UW hospital have
> fortunately signed their contract that is good until the reopener in
> 1013 and we are not strictly state employees. However, the way things
> are looking, it is not out of the question that he may also try to void
> our contracts. Our pay MAY go up, but we will no longer have much, if
> anything, for benefits. Don't count on sending your kids to the UW
> colleges if this goes through.
> Sorry, I'm fighting mad right now. I'm not usually big on the unions,
> but this is HUGE for millions of Wisconsinites. GGRRRRR!
> Claire
>
> Were there any other Wisconsin techs at the rally today? Even the
> Police and Firefighters unions were down there!
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Michael
> Hillmer
> Sent: Tue 2/15/2011 5:02 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Wage question for Wisconsin
>
>
>
> We are dermatology clinic in Northeast Wisconsin and we are trying to do
> gather accurate wage data. Can anybody offer wages for an HT, HtL and
> lab assistants? Any help would be greatly appreciated.
>
>
> Thank you-
>
>
>
> Michael Hillmer PHR
>
> HR Coordinator
>
> Dermatology Associates of Wisconsin
>
> Phone: (920)683-5278
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 16 Feb 2011 08:59:44 -0600
> From: <sgoebel <@t> mirnarx.com>
> Subject: RE: [Histonet] Section position on slides
> To: <tjey <@t> hotmail.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D957F2A7D21959488C492A2680F9920A1C58F5 <@t> svrexch.asuragen.us>
> Content-Type: text/plain; charset="US-ASCII"
>
> I learned from an old school HT and studied using the Carson book. In
> this book there are several pages on where to put sections. If you
> don't have the Frieda Carson book (or "the bible" as I refer to it), get
> it. A new edition just came out about a year ago. If you're an ASCP
> member I think the price is reduced...
> For the most part, just put everything as close to the middle of the
> clear glass part of the slide as possible. This also does well for IHC
> automation. I think most automated machines you can adjust the drop
> range too if you want to put the sections on the bottom or top?
> Good Luck!
>
> Sarah Goebel, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya
> Ewing-Finchem
> Sent: Tuesday, February 15, 2011 11:09 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Section position on slides
>
>
> I am trying to put together a training document around microtomy and
> sectioning and am finding it hard to find information around the
> placement of the actual sections on the slides. These are the
> objectives I am looking to answer. Is this information found in any
> publications?
>
> 1) Tissue / Section Placement: Are there published guidelines /
> documentation on precisely where you should place tissue sections on a
> 25mm x 75mm glass slide? Perhaps more importantly, where you should
> NOT place tissue (ie. "x" mm from the edge of the glass slide)?
>
> 2) Diagnosable Slide Staining Area: With automation becoming more
> widely used in IHC, are there published guidelines / documentation on
> the usable or diagnosable staining area on a 25mm x 75mm glass slide?
> For instance, would you define that as the area under a traditional
> coverslip? Would this be defined as the entire slide below the label?
> Or is this some distance from all the edges of the slide? With some
> automated systems, it is near impossible to get edge to edge staining.
> Is this acceptable?
>
>
> Thanks for any ideas.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 16 Feb 2011 10:15:29 -0500
> From: "Gill, Caula A." <cgill <@t> marylandgeneral.org>
> Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis
> To: "Ingles Claire " <CIngles <@t> uwhealth.org>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 087A9911BBAFDE4B8151CB148586E2C23A9F38 <@t> MDGEN-EXCH1.marylandgeneral.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I agree, gum sounds better not to mention the taste
> CG
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
> Claire
> Sent: Tuesday, February 15, 2011 5:55 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis
>
> Wouldn't GUM be a more palatable option? Who knows where some of those
> rubber bands have been!
> Claire
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Robert
> Richmond
> Sent: Mon 2/14/2011 11:09 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: saliva for glycogen hydrolysis
>
>
>
> Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
> spit method (which by the way is still how it's done by the small
> pathology services I work on):
>
> You can produce copious quantities of saliva by the simple expedient of
> chewing on a rubber band for a couple of minutes. This used to be the
> technique used to obtain saliva samples for determination of ABH
> substance secretor status in the blood bank. (I belong to that elite 20%
> of donors who are non-secretors, and Lewis-a positive to prove
> it.)
>
> Bob Richmond
> Samurai pathologist and occasional sialogogue Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 14
> Date: Wed, 16 Feb 2011 10:25:27 -0500
> From: Victoria Baker <bakevictoria <@t> gmail.com>
> Subject: Re: [Histonet] Wage question for Wisconsin
> To: Michael Hillmer <mhillmer <@t> dermwisconsin.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <AANLkTinj-abey-jrMWh2MjtKe+YWj4oqJwtwNW4+Cx6K <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Michael,
>
> ASCP has a wage/salary survey that they distribute based upon region and
> certification. Some have said that this is biased as not everyone does
> participate. I tend to agree with the survey, but also recognize that
> dermatopathology is a 'specialty' type lab. I worked in Dermpath at NYU
> back in the 80's to early 90's and our salaries were not identical to the
> clinical labs as we were a part of the medical school and not the hospital.
> Yes the hospital was 1199 at that time, I cannot say if they are still.
>
> My recommendation is to go with what you can find from the survey, but also
> network with other dermpath or other specialty labs in your area that you
> may know or who you can connect with through others - either on the
> listserver or off - to possibly get a better sounding of salaries. HR at
> your hospital might be also able to help if you have someone there that you
> can work with and trust to obtain some of the data you're looking for and
> understands what your specific needs are within the lab. I would include
> your pathologists in this as much as possible as they will be needed to
> support your request(s) as they are essentially your clients.
>
> Vikki
>
> On Tue, Feb 15, 2011 at 6:02 PM, Michael Hillmer <
> mhillmer <@t> dermwisconsin.com
> > wrote:
>
> > We are dermatology clinic in Northeast Wisconsin and we are trying to do
> > gather accurate wage data. Can anybody offer wages for an HT, HtL and
> > lab assistants? Any help would be greatly appreciated.
> >
> >
> >
> >
> >
> >
> >
> >
> >
> > Thank you-
> >
> >
> >
> > Michael Hillmer PHR
> >
> > HR Coordinator
> >
> > Dermatology Associates of Wisconsin
> >
> > Phone: (920)683-5278
> >
> > Fax: (920)686-9674
> >
> > Cell: (920)860-6360
> >
> >
> >
> > The materials and information in this e-mail are confidential and may
> > contain Protected Health Information covered under the HIPAA Privacy
> > Rule. If you are not the intended recipient, be advised that any
> > unauthorized use, disclosure, copying, distribution, or action taken in
> > reliance on the contents of this information is strictly forbidden by
> > law. If you have received this e-mail in error, please notify me by
> > reply e-mail and then delete this message. Do not pass any of this
> > information to anyone else. Thank you for your cooperation.
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 15
> Date: Wed, 16 Feb 2011 10:27:55 -0500
> From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
> Subject: [Histonet] Correction, Correction on cutoff date for GSH
> meeting
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9BF995BC0E47744E9673A41486E24EE22DBB3A7DE3 <@t> MERCERMAIL.MercerU.local
> >
> Content-Type: text/plain; charset="us-ascii"
>
> Correction: There is a correction on the cutoff date for the hotel
> reservations. Cutoff date for discounted room rate is February 23rd, Not
> March 1st. There are several other events going on that weekend and the
> hotel is filling up fast. Please make your reservations and register soon
> so you will not miss out on the great room rate and a great meeting.
>
> Shirley Powell
>
>
> Hi Georgia, Alabama, ALL histotechs,
>
>
>
> The Georgia Society for Histotechnology invites you to our meeting March
> 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near
> Columbus, Ga. and very convenient to Alabama folks, so come across the line.
> The invitation extends to any other states as well. Callaway Gardens is a
> fantastic site for family vacations, golf lovers, nature lovers, so come to
> Georgia for a visit and take in a wealth of histology knowledge.
>
>
>
> The deadline for making hotel reservations is March 1, 2011 so that gives
> you a month to make your plans to attend, don't delay. The Mountain Creek
> Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can
> call for hotel reservations at 1-800-225-5292. Room rates start at $99
> which includes Continental Breakfast and Admission to the Park. For more
> information about things to do at Callaway click on the link here:
> http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx
>
>
>
> Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The
> complete program can be downloaded from our website at this link:
> www.histosearch.com/gsh<http://www.histosearch.com/gsh<
> http://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh>> then
> click on GSH symposium link at the bottom of the home page. There you will
> find the complete program with registration form. The vendor registration
> form is on the same page for any last minute vendors who want to exhibit at
> our meeting. If anyone has questions, please contact me for assistance.
>
>
>
> Come TRANSFORM yourselves.
>
>
>
>
>
> Shirley Powell
>
> GSH Secretary
>
>
> Shirley A. Powell, HT(ASCP)HTL, QIHC
> Technical Director
> Histology Curricular Support Laboratory
> Mercer University School of Medicine
> 1550 College Street
> Macon, GA 31207
> 478-301-2374 Lab
> 478-301-5489 Fax
>
>
>
> ------------------------------
>
> Message: 16
> Date: Wed, 16 Feb 2011 08:59:36 -0700
> From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
> Subject: [Histonet] Pathco double-blade handle
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 4D14F0FC9316DD41972D5F03C070908B02E4766A <@t> nmdamailsvr.nmda.ad.nmsu.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Am looking for this item to replace one that was older than I am. I
> need the handle (perhaps Pathco #D2877) for the blades (Pathco
> #D2878-2C). The catch is that I want the SOLID BRASS handle - not the
> aluminum. I've "googled" all over the place - can anyone help me find
> these handles? Muchas gracias!
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM 87102
>
> 505-383-9278 (Histology Lab)
>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Wed, 16 Feb 2011 11:09:31 -0500
> From: "jstaruk" <jstaruk <@t> masshistology.com>
> Subject: RE: [Histonet] Pathco double-blade handle
> To: "'Breeden, Sara'" <sbreeden <@t> nmda.nmsu.edu>, "'Histonet'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <005501cbcdf3$e623bd40$b26b37c0$@masshistology.com>
> Content-Type: text/plain; charset="us-ascii"
>
> I've found two brass handles on Ebay in the last few years. I don't know
> if
> you're aware of this but the exact same blades that fit these handles can
> be
> purchased at Home Depot in the carpet section. $10 for 50 of them.
>
>
>
> Jim
>
>
>
> _______________________
>
> James E. Staruk HT(ASCP)
>
> <http://www.masshistology.com> www.masshistology.com
>
> www.nehorselabs.com
>
>
>
>
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
> Sara
> Sent: Wednesday, February 16, 2011 11:00 AM
> To: Histonet
> Subject: [Histonet] Pathco double-blade handle
>
>
>
> Am looking for this item to replace one that was older than I am. I
> need the handle (perhaps Pathco #D2877) for the blades (Pathco
> #D2878-2C). The catch is that I want the SOLID BRASS handle - not the
> aluminum. I've "googled" all over the place - can anyone help me find
> these handles? Muchas gracias!
>
>
>
> Sally Breeden, HT(ASCP)
>
> New Mexico Department of Agriculture
>
> Veterinary Diagnostic Services
>
> 1101 Camino de Salud NE
>
> Albuquerque, NM 87102
>
> 505-383-9278 (Histology Lab)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _____
>
> No virus found in this message.
> Checked by AVG - www.avg.com
> Version: 10.0.1204 / Virus Database: 1435/3447 - Release Date: 02/16/11
>
>
>
> ------------------------------
>
> Message: 18
> Date: Wed, 16 Feb 2011 11:12:00 -0500
> From: "caymanfleck <@t> gmail.com" <caymanfleck <@t> gmail.com>
> Subject: [Histonet] Exam Prep
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <AANLkTimyreMDoNo+U3ApAsbhJZPnhstLqxCDyarPo+61 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello Histoland,
>
> I'm preparing for the HTL exam after being trained on-the-job and working
> in
> histo for a few years. I'm using the standard 'Theory and practice...'
> text
> as my main resource. I'm wondering if anyone has used either the ASCP
> practice tests (a set of 5 are available from ascp for about $30) or the
> NSH
> Self-assessment Program (which has modules that correspond to the exam
> sections). The NSH program is expensive at about $240 but I don't know if
> it's worth it. Has anyone used either of these resources?
>
> Thanks!
>
>
> ------------------------------
>
> Message: 19
> Date: Wed, 16 Feb 2011 10:23:52 -0600
> From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
> Subject: RE: [Histonet] Exam Prep
> To: <caymanfleck <@t> gmail.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 6DBD71C31D7E444482E5D3DFBC202D260245D539 <@t> txhous1eb012.uson.usoncology.int>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I just used the Freida Carson Histotechnology A Self-Instructional Test.
>
>
>
> Thanks,
>
> Nacaela Johnson, HTL (ASCP)
> Histotechnologist
> KCCC Pathology
> 12000 110th St., Ste. 400
> Overland Park, KS 66210
> Office: 913-234-0576
> Fax: 913-433-7639
> Email: Nacaela.Johnson <@t> USOncology.com
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> caymanfleck <@t> gmail.com
> Sent: Wednesday, February 16, 2011 10:12 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Exam Prep
>
> Hello Histoland,
>
> I'm preparing for the HTL exam after being trained on-the-job and
> working in histo for a few years. I'm using the standard 'Theory and
> practice...' text as my main resource. I'm wondering if anyone has used
> either the ASCP practice tests (a set of 5 are available from ascp for
> about $30) or the NSH Self-assessment Program (which has modules that
> correspond to the exam sections). The NSH program is expensive at about
> $240 but I don't know if it's worth it. Has anyone used either of these
> resources?
>
> Thanks!
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> </pre>The contents of this electronic mail message and any attachments are
> confidential, possibly privileged and intended for the addressee(s)
> only.<br>Only the addressee(s) may read, disseminate, retain or otherwise
> use this message. If received in error, please immediately inform the sender
> and then delete this message without disclosing its contents to
> anyone.</pre>
>
>
>
>
> ------------------------------
>
> Message: 20
> Date: Wed, 16 Feb 2011 10:35:21 -0600
> From: <sgoebel <@t> mirnarx.com>
> Subject: RE: [Histonet] Exam Prep
> To: <Nacaela.Johnson <@t> USONCOLOGY.COM>, <caymanfleck <@t> gmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D957F2A7D21959488C492A2680F9920A1C5906 <@t> svrexch.asuragen.us>
> Content-Type: text/plain; charset="US-ASCII"
>
> If you basically memorize this book, and then have a pretty good
> understanding of IHC...then you should do fine.
>
> Sarah Goebel, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson,
> Nacaela
> Sent: Wednesday, February 16, 2011 10:24 AM
> To: caymanfleck <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Exam Prep
>
> I just used the Freida Carson Histotechnology A Self-Instructional Test.
>
>
>
> Thanks,
>
> Nacaela Johnson, HTL (ASCP)
> Histotechnologist
> KCCC Pathology
> 12000 110th St., Ste. 400
> Overland Park, KS 66210
> Office: 913-234-0576
> Fax: 913-433-7639
> Email: Nacaela.Johnson <@t> USOncology.com
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> caymanfleck <@t> gmail.com
> Sent: Wednesday, February 16, 2011 10:12 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Exam Prep
>
> Hello Histoland,
>
> I'm preparing for the HTL exam after being trained on-the-job and
> working in histo for a few years. I'm using the standard 'Theory and
> practice...' text as my main resource. I'm wondering if anyone has used
> either the ASCP practice tests (a set of 5 are available from ascp for
> about $30) or the NSH Self-assessment Program (which has modules that
> correspond to the exam sections). The NSH program is expensive at about
> $240 but I don't know if it's worth it. Has anyone used either of these
> resources?
>
> Thanks!
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> </pre>The contents of this electronic mail message and any attachments
> are confidential, possibly privileged and intended for the addressee(s)
> only.<br>Only the addressee(s) may read, disseminate, retain or
> otherwise use this message. If received in error, please immediately
> inform the sender and then delete this message without disclosing its
> contents to anyone.</pre>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 21
> Date: Wed, 16 Feb 2011 11:08:07 -0600
> From: "Bill B." <bill501 <@t> mindspring.com>
> Subject: Re: [Histonet] Section position on slides
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <p06240801c981b14a90ef@[4.244.63.90]>
> Content-Type: text/plain; charset="iso-8859-1"
>
> As a pathologist I have some preferences on how certain tissues are
> oriented on a slide and work with my histotechs so they can learn my prefs.
> I also like my slides in certain orientation in the slide tray. EG, for
> orientation of skin, I like the the dermis at the top when I am looking at
> the slide. Some other tissues can be so oriented, some cannot. We often put
> multiple levels on one slide and I would prefer them to be all oriented
> similarly and in a straight line.
>
> The most annoying thing I see beyond coverslipping the wrong side, is the
> label covering a part of the specimen. I don't care how close to the edge a
> section is as long as it is all on the slide and covered by the cover-slip
> or mounting medium.
>
> I have no idea is these preferences are weird. My point is the answer to
> you 1st question may depend on your particular pathologists' preferences.
>
> BIll Blank
>
> At 10:09 PM -0700 2/15/11, Tanya Ewing-Finchem wrote:
> >1) Tissue / Section Placement: Are there published guidelines /
> documentation on precisely where you should place tissue sections on a 25mm
> x 75mm glass slide? Perhaps more importantly, where you should NOT place
> tissue (ie. ?x? mm from the edge of the glass slide)?
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Wed, 16 Feb 2011 17:20:57 +0000
> From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
> Subject: RE: [Histonet] Section position on slides
> To: Tanya Ewing-Finchem <tjey <@t> hotmail.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DF1CBA3D83D9A344A7D6A045188E448405A9A2 <@t> EMBX-CLFT1.cdc.gov>
> Content-Type: text/plain; charset="us-ascii"
>
> I always try to center the section equally from sides and top-bottom as
> possible. This means measure from the bottom of the frosted edge as the
> "top". The Artisan special stains system has a clip that attaches around
> the slide to allow reagents to pool onto the sections and incubate. If the
> section is too close to the sides then these areas do not stain adequately.
> Sometimes if the tissue section itself is very large this is unavoidable.
> With automated coverslippers you must also consider placement of tissue to
> allow for proper coverage.
>
> If I am cutting multiple unstained slides for subsequent testing I try to
> orient the tissue the same on each slide to facilitate the reading of these
> slides by the pathologist's
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya
> Ewing-Finchem
> Sent: Wednesday, February 16, 2011 12:09 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Section position on slides
>
>
> I am trying to put together a training document around microtomy and
> sectioning and am finding it hard to find information around the placement
> of the actual sections on the slides. These are the objectives I am looking
> to answer. Is this information found in any publications?
>
> 1) Tissue / Section Placement: Are there published guidelines /
> documentation on precisely where you should place tissue sections on a 25mm
> x 75mm glass slide? Perhaps more importantly, where you should NOT place
> tissue (ie. "x" mm from the edge of the glass slide)?
>
> 2) Diagnosable Slide Staining Area: With automation becoming more widely
> used in IHC, are there published guidelines / documentation on the usable or
> diagnosable staining area on a 25mm x 75mm glass slide? For instance, would
> you define that as the area under a traditional coverslip? Would this be
> defined as the entire slide below the label? Or is this some distance from
> all the edges of the slide? With some automated systems, it is near
> impossible to get edge to edge staining. Is this acceptable?
>
>
>
> Thanks for any ideas.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 23
> Date: Wed, 16 Feb 2011 12:27:32 -0500
> From: Helen Fedor <hfedor <@t> jhmi.edu>
> Subject: RE: [Histonet] Section position on slides
> To: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>, Tanya
> Ewing-Finchem <tjey <@t> hotmail.com>, "
> histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> DC2D3533BC05784793C2FCE01FCFB9260539A47E2B <@t> JHEMTEXVS3.win.ad.jhu.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Since automation is becoming more and more a part of all Histology labs the
> demands of placement of the tissue on the slides varies for different
> instruments. Stainers, coverslippers and now with slide scanning as well. So
> I do not believe that there is a silver bullet answer.
>
> Helen L. Fedor
>
> Tissue Microarray Lab, Manager
> Prostate Spore Lab, Manager
> Johns Hopkins University
> 600 N. Wolfe St,?| Marburg Room 406
> Baltimore, MD?| 21287-7065
>
> 410.614.1660
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine
> (CDC/OID/NCEZID)
> Sent: Wednesday, February 16, 2011 12:21 PM
> To: Tanya Ewing-Finchem; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Section position on slides
>
> I always try to center the section equally from sides and top-bottom as
> possible. This means measure from the bottom of the frosted edge as the
> "top". The Artisan special stains system has a clip that attaches around
> the slide to allow reagents to pool onto the sections and incubate. If the
> section is too close to the sides then these areas do not stain adequately.
> Sometimes if the tissue section itself is very large this is unavoidable.
> With automated coverslippers you must also consider placement of tissue to
> allow for proper coverage.
>
> If I am cutting multiple unstained slides for subsequent testing I try to
> orient the tissue the same on each slide to facilitate the reading of these
> slides by the pathologist's
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya
> Ewing-Finchem
> Sent: Wednesday, February 16, 2011 12:09 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Section position on slides
>
>
> I am trying to put together a training document around microtomy and
> sectioning and am finding it hard to find information around the placement
> of the actual sections on the slides. These are the objectives I am looking
> to answer. Is this information found in any publications?
>
> 1) Tissue / Section Placement: Are there published guidelines /
> documentation on precisely where you should place tissue sections on a 25mm
> x 75mm glass slide? Perhaps more importantly, where you should NOT place
> tissue (ie. "x" mm from the edge of the glass slide)?
>
> 2) Diagnosable Slide Staining Area: With automation becoming more widely
> used in IHC, are there published guidelines / documentation on the usable or
> diagnosable staining area on a 25mm x 75mm glass slide? For instance, would
> you define that as the area under a traditional coverslip? Would this be
> defined as the entire slide below the label? Or is this some distance from
> all the edges of the slide? With some automated systems, it is near
> impossible to get edge to edge staining. Is this acceptable?
>
>
>
>
> Thanks for any ideas.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 87, Issue 28
> ****************************************
>
> This e-mail message (including any attachments) is for the sole use of
> the intended recipient(s) and may contain confidential and privileged
> information. If the reader of this message is not the intended
> recipient, you are hereby notified that any dissemination, distribution
> or copying of this message (including any attachments) is strictly
> prohibited.
>
> If you have received this message in error, please contact
> the sender by reply e-mail message and destroy all copies of the
> original message (including attachments).
>
>
>
> ------------------------------
>
> Message: 12
> Date: Thu, 17 Feb 2011 16:46:04 +0200
> From: "Gil Sharon" <gilsharo <@t> post.tau.ac.il>
> Subject: [Histonet] Drosophila Sectioning
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4BD4759CE4D84480B0D73E317744289B <@t> danas>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi All,
>
>
>
> As a microbial ecologist, working with drosophila, I'm looking for a couple
> of protocols (or papers) for fixation and paraffin-embedding of embryos,
> 3rd
> instar larvae, pupae and adult D. melanogaster (these sections will later
> be
> used for fluresecnt-in-situ-hybridization probing for specific bacteria).
> Looking in the literature, I could only find protocols for embryos (or fly
> heads). As I'm really new to histology, I hope someone here will be able to
> help me with this. I have the help of a histo lab here, but they're working
> on mice, so no experience with drosophila (or insects at all).
>
>
>
> Thanks in advance,
>
> Gil.
>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Thu, 17 Feb 2011 07:49:53 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP
> section
> To: "'Amy Lee'" <amylee779 <@t> yahoo.com>, "'histonet'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <3A5E8FD47DF948B888D5F8765F834905 <@t> prueggihctechlt>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I do not know of a neutrophil specific ab for human ffpe tissue. You could
> do just DAB without blocking with h202 for myleoperoxidase but it will not
> distinguish between eosinophils and neutrophils myeloperoxidase will stain
> all myeloid cells with granules in the cytoplasm (granulocytes).
>
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Lee
> Sent: Wednesday, February 16, 2011 1:07 PM
> To: histonet
> Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
>
> Hello,
> I was asked to perform neutrophil IHC on rat FFP sections. I searched
> around
> and found most are for mouse tissue. There is one from Serotec mouse anti
> rat but it does not work on paraffin section.
>
> Could anybody recommend a good antibody?
>
> Thanks in advance,
>
> Amy
>
>
>
>
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> End of Histonet Digest, Vol 87, Issue 30
> ****************************************
>
--
Damien Laudier
Laudier Histology
(917) 836-7573
www.Laudierhistology.com <http://www.LaudierArt.com>
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