[Histonet] Previously Cut Slides for FS on Muscle

[Mitchell Jean A]

Did you run an H&E or any other stain on the case before you stored the
slides at -80?  The muscle tissue very likely was dried out and had
artifact to begin with even before you stored the sections/slides in the
freezer.  

I have stored muscle sections for days/weeks/months at -80 without any
adverse staining issues.

Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital & Clinics
Neuromuscular Laboratory
600 Highland Avenue
Madison, WI  53792-5132 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
Allison
Sent: Monday, February 14, 2011 10:11 AM
To: histonet
Cc: akallison <@t> chla.usc.edu
Subject: [Histonet] Previously Cut Slides for FS on Muscle

Happy Valentine's week!

I haven't done histochemistry on muscle biopsies since the early 80's.
I am using a isopentane/ liquid nitrogen method to freeze the muscle
biopsy, so that part is covered.  I cut several practice  
slides on a post the previous night to work up my stain protocol's.   
I want to optimize our stains so I cut around 25 slides at a time. I put
them in a plastic slide box, wrapped it in aluminum foil, and then put
it in the -80 freezer till the next day.  Sounded good, but the tissue
dried out, and had artifact.

Obviously -80 was not good for the slides.  Can I cut the slides and
leave them at room temperature till the next day, or should I put them
in a -20 freezer? I am curious how long slides which have been cut on
muscle Bx's will last at RT.  Anyone know, or have any suggestions?

Thank you in advance for your assistance, Akemi Allison BS, HT (ASCP)
HTL

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