[Histonet] Re: saliva for glycogen hydrolysis

Robert Richmond rsrichmond <@t> gmail.com
Mon Feb 14 11:09:19 CST 2011

Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
spit method (which by the way is still how it's done by the small
pathology services I work on):

You can produce copious quantities of saliva by the simple expedient
of chewing on a rubber band for a couple of minutes. This used to be
the technique used to obtain saliva samples for determination of ABH
substance secretor status in the blood bank. (I belong to that elite
20% of donors who are non-secretors, and Lewis-a positive to prove

Bob Richmond
Samurai pathologist and occasional sialogogue
Knoxville TN

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