[Histonet] Previously Cut Slides for FS on Muscle
Akemi Allison
akemiat3377 <@t> yahoo.com
Mon Feb 14 10:11:10 CST 2011
Happy Valentine's week!
I haven't done histochemistry on muscle biopsies since the early
80's. I am using a isopentane/ liquid nitrogen method to freeze the
muscle biopsy, so that part is covered. I cut several practice
slides on a post the previous night to work up my stain protocol's.
I want to optimize our stains so I cut around 25 slides at a time. I
put them in a plastic slide box, wrapped it in aluminum foil, and
then put it in the -80 freezer till the next day. Sounded good, but
the tissue dried out, and had artifact.
Obviously -80 was not good for the slides. Can I cut the slides and
leave them at room temperature till the next day, or should I put
them in a -20 freezer? I am curious how long slides which have been
cut on muscle Bx's will last at RT. Anyone know, or have any
suggestions?
Thank you in advance for your assistance,
Akemi Allison BS, HT (ASCP) HTL
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