[Histonet] Previously Cut Slides for FS on Muscle

Akemi Allison akemiat3377 <@t> yahoo.com
Mon Feb 14 10:11:10 CST 2011

Happy Valentine's week!

I haven't done histochemistry on muscle biopsies since the early  
80's.  I am using a isopentane/ liquid nitrogen method to freeze the  
muscle biopsy, so that part is covered.  I cut several practice  
slides on a post the previous night to work up my stain protocol's.   
I want to optimize our stains so I cut around 25 slides at a time. I  
put them in a plastic slide box, wrapped it in aluminum foil, and  
then put it in the -80 freezer till the next day.  Sounded good, but  
the tissue dried out, and had artifact.

Obviously -80 was not good for the slides.  Can I cut the slides and  
leave them at room temperature till the next day, or should I put  
them in a -20 freezer? I am curious how long slides which have been  
cut on muscle Bx's will last at RT.  Anyone know, or have any  

Thank you in advance for your assistance,
Akemi Allison BS, HT (ASCP) HTL

More information about the Histonet mailing list