[Histonet] RE: saliva for glycogen hydrolysis

[Tony Henwood]

" and few books seem to provide that information"

Probably because it is so disgusting!

We used to SPIT on the sections to cover, incubate at room temp for 10 min and rinse well in water.
If we had too many slides to do (I could probably SPIT on 3 slides before I dried up) I would spit into a 50ml specimen container and add PBS buffer, equal volumes, mix and pipette on to the slides and incubate for 10-15 min at room temp.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils, Paul
Sent: Saturday, 12 February 2011 9:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] saliva for glycogen hydrolysis

Those who use human saliva rather than diastase - for how long do you apply the saliva?  At what temperature?  Do you cover the saliva and section with a coverslip during treatment?  I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information.  Thanks.

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