[Histonet] RE: saliva for glycogen hydrolysis

Tony Henwood AnthonyH <@t> chw.edu.au
Sun Feb 13 17:12:42 CST 2011

" and few books seem to provide that information"

Probably because it is so disgusting!

We used to SPIT on the sections to cover, incubate at room temp for 10 min and rinse well in water.
If we had too many slides to do (I could probably SPIT on 3 slides before I dried up) I would spit into a 50ml specimen container and add PBS buffer, equal volumes, mix and pipette on to the slides and incubate for 10-15 min at room temp.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils, Paul
Sent: Saturday, 12 February 2011 9:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] saliva for glycogen hydrolysis

Those who use human saliva rather than diastase - for how long do you apply the saliva?  At what temperature?  Do you cover the saliva and section with a coverslip during treatment?  I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information.  Thanks.

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list