[Histonet] RE: saliva for glycogen hydrolysis

[Anthony Reilly]

Hi Tony
 
We used to do something similar.  Spit into a test tube, add equal volume of distilled water, put on the lid and shake vigorously which makes the solution more liquid and easier to use. 
 
regards
Tony
 
 
 

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
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Clinical and Statewide Services Division| QueenslandHealth
 
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t> health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/
 
 


>>> Tony Henwood <AnthonyH <@t> chw.edu.au> 14/02/2011 9:12 am >>>
" and few books seem to provide that information"

Probably because it is so disgusting!

We used to SPIT on the sections to cover, incubate at room temp for 10 min and rinse well in water.
If we had too many slides to do (I could probably SPIT on 3 slides before I dried up) I would spit into a 50ml specimen container and add PBS buffer, equal volumes, mix and pipette on to the slides and incubate for 10-15 min at room temp.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils, Paul
Sent: Saturday, 12 February 2011 9:42 AM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] saliva for glycogen hydrolysis

Those who use human saliva rather than diastase - for how long do you apply the saliva?  At what temperature?  Do you cover the saliva and section with a coverslip during treatment?  I used to do this many years ago, but have been using diastase for so long now that I don't remember the particulars of the saliva method, and few books seem to provide that information.  Thanks.

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