[Histonet] H&E stain uneven

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Feb 11 13:54:57 CST 2011

Check your dewaxing → hydrating protocol and change reagents frequently.
René J.

--- On Fri, 2/11/11, Madeline Gi <madelinegi <@t> yahoo.com> wrote:

From: Madeline Gi <madelinegi <@t> yahoo.com>
Subject: [Histonet] H&E stain uneven
To: Histonet <@t> lists.utsouthwestern.edu
Date: Friday, February 11, 2011, 1:44 PM

Hello everyone in the histology world, I have a problem with my H&E stain this just started and I am not sure why.  Can someone tell me how does a routine H&E stain unevenly?  I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated….  

Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelinegi <@t> yahoo.com

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