[Histonet] Help with OCT problem

Ingles Claire CIngles <@t> uwhealth.org
Thu Feb 10 16:47:48 CST 2011

Same with ETOH. We wipe our surfaces down with it to clean them so no cross contamination as we freeze our skin here on the metal side areas of our cryostats. If the alcohol hasn't been wiped dry or evaporated we have that problem too.


From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Liz Chlipala
Sent: Thu 2/10/2011 1:49 PM
To: Reynolds,Donna M; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with OCT problem


I have seen soft or rather sticky OCT samples in the past.  If you
freeze in isopentane and don't let the isopentane evaporate off the
samples prior to wrapping the sample in foil, that excess isopentane
changes the OCT, it makes it sticky.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060

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