[Histonet] Help with OCT problem

[Ingles Claire]

Same with ETOH. We wipe our surfaces down with it to clean them so no cross contamination as we freeze our skin here on the metal side areas of our cryostats. If the alcohol hasn't been wiped dry or evaporated we have that problem too.
Claire

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Liz Chlipala
Sent: Thu 2/10/2011 1:49 PM
To: Reynolds,Donna M; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Help with OCT problem



Donna

I have seen soft or rather sticky OCT samples in the past.  If you
freeze in isopentane and don't let the isopentane evaporate off the
samples prior to wrapping the sample in foil, that excess isopentane
changes the OCT, it makes it sticky.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com