[Histonet] Help with OCT problem

[Patrick Laurie]

Donna,
I would also check to make sure that the tissue wasn't held in an
something with a lot of alcohol, like RNA later.  I found out first
hand that it doesn't work well.  Good luck

On Thu, Feb 10, 2011 at 11:38 AM, Reynolds,Donna M
<dreynold <@t> mdanderson.org> wrote:
> Has anyone ever experienced OCT blocks that cut like they are soft. I have turned the cyrostat down 10 degrees lower than usual and they are still soft. Really weird part is that I have 20 blocks in this group and some are soft and others are just fine. Tried changing angle, new blade nothing helps. When I finally manage to get a decent section and pick it up on the slide, the OCT around the tissue want even pick up smooth it wrinkles really bad.
> I tried just a block of OCT that I froze on a chuck and it cut great.  I am clueless as to what is going on.
> Samples were frozen by someone else and brought to me to be cut.
> Donna Reynolds Core IHC Lab
> M.D. Anderson Cancer Center, Houston TX
> Research lab
> 713-192-8106.
>
>
> Donna Reynolds Core IHC Lab
> Dept. Cancer Biology, SRB 1.660
> 713-792-8106
>
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com