[Histonet] Benchmark Problems
jnocito <@t> satx.rr.com
Wed Feb 9 18:01:56 CST 2011
I would also see if Ventana can send you some test slides to test the
heating. At my old lab, we had a problem with the heating plates. They
repaired the entire platform. Just a thought.
----- Original Message -----
From: "Mark Tarango" <marktarango <@t> gmail.com>
To: "Dessoye, Michael J" <mjdessoye <@t> wvhcs.org>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 09, 2011 10:58 AM
Subject: Re: [Histonet] Benchmark Problems
> Hi Mike,
> Did you wash the bottles and rinse them and then pump the water through
> instrument before adding reagents and purging all again? We had a problem
> like this about 2 weeks ago. I think someone loaded the wrong bulk
> onto the instrument. Cleaning it, purging with water and adding newly
> bulk reagents fixed the problem for us.
> On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J
> <mjdessoye <@t> wvhcs.org>wrote:
>> We started having a strange problem with our Ventana Benchmark XT. Out
>> the blue, our slides stopped staining. With most antibodies we get no
>> reaction, some that were very strongly positive are now only very lightly
>> staining. They do appear to counterstain OK. Following Ventana's
>> recommendations we completely changed out all of our bulk fluids, purged
>> lines, changed antibodies and DAB kits. The last shot is some kind of
>> instrument issue that's not triggering an error. Anyone ever see
>> like this?
>> Mike Dessoye
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