[Histonet] Benchmark Problems

[Mark Tarango]

Hi Mike,

Did you wash the bottles and rinse them and then pump the water through the
instrument before adding reagents and purging all again?  We had a problem
like this about 2 weeks ago.  I think someone loaded the wrong bulk reagent
onto the instrument.  Cleaning it, purging with water and adding newly made
bulk reagents fixed the problem for us.

Mark

On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J <mjdessoye <@t> wvhcs.org>wrote:

> Hello,
>
> We started having a strange problem with our Ventana Benchmark XT.  Out of
> the blue, our slides stopped staining.  With most antibodies we get no
> reaction, some that were very strongly positive are now only very lightly
> staining.  They do appear to counterstain OK.  Following Ventana's
> recommendations we completely changed out all of our bulk fluids, purged the
> lines, changed antibodies and DAB kits.  The last shot is some kind of
> instrument issue that's not triggering an error.  Anyone ever see anything
> like this?
>
> Mike Dessoye
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