[Histonet] Her2 Fixation Requirement

Kim.Donadio <@t> bhcpns.org Kim.Donadio <@t> bhcpns.org
Fri Feb 4 11:13:52 CST 2011

We adhere the ink with bouins sol'n. Have had no problems. 

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

"Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/03/2011 04:54 PM

"Weems, Joyce" <JWeems <@t> sjha.org>, "Paula Lucas" <plucas <@t> biopath.org>, 
<histonet <@t> lists.utsouthwestern.edu>

RE: [Histonet] Her2 Fixation Requirement

Does anyone have issues with inking the specimen after it has been placed 
in formalin?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Weems,
Sent: Wednesday, February 02, 2011 12:59 PM
To: Paula Lucas; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement 

We have the nurse document the time of removal on the requisition. The 
next requisition update will have a spot for this. They have been very 
cooperative and do a good job. 

In addition, you must document the "cold ischmic time" - that is the time 
from removal until time in formalin. This is important when the specimen 
goes for xray or whatever. So there is a removal time, an into formalin 
time and an out of formalin time. 

Then if we don't have time allowed to meet the fixation time with the 
regular, it is put on a late processor, if we have one available, or it is 
held overnight. And if it has to come off on Sunday, we have a med tech 
remove it from the processor and it waits for us to come on Monday to 
embed it. 

The pathologists document all this time in the report. 

Best! J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula 
Sent: Wednesday, February 02, 2011 12:11
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Her2 Fixation Requirement 

Hello histoland
I was just given the task to find a solution that is easy and will also 
comply with the CAP guideline for formalin fixation documentation, and so 
I started my research on the Histonet archives.  I found some good 
information, but was hoping to get more feedback. 
Would you mind sharing with me the actions you are taking to comply with 
the guideline? 
We are a private lab and we provide histology/pathology service for 2 
hospitals and a few surgery centers.  We send our blocks to Genzyme for 
Her2, and we must document on their test order sheet how many hours the 
tissues have been fixed in formalin.
I'm assuming I will need to start keeping a log here, with documentation 
that shows what time the tissue was excised and placed in formalin from 
the OR, and then documentation that shows the time it was dissected and 
then placed in the tissue processor. 
The problem that I may come across is getting the OR nurse to document the 
time for us.  I don't know...maybe we need to put another sections on our 
requisition form, or maybe something on the formalin container itself for 
the nurse to write on.  It'll be a hassle at first but if I can get the 
hospitals lab director involved, I'm sure it will work itself out.
Anyway, if you wouldn't mind sharing some of your ideas, I would really 
appreciate it.
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA


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