[Histonet] 2% low melting agarose gel

Boyce, Bobbie bboyce <@t> NEMOURS.ORG
Fri Dec 16 06:12:33 CST 2011


Hello,

Has anyone had any experience with cutting cell mixed with 2% low
melting agarose gel? The cells were fixed in 10% NBF, mixed with the gel
and then processed to a paraffin block.

I've tried a couple of techniques, but the end result is mush.

Any ideas or suggestions?


Bobbie Boyce
Histology Specialist III
duPont Hospital for Children
Biomedicla Research, ARB 264
1600 Rockland Road
Wilmington, DE 19803
302-651-6771 (Lab)
302-651-5010 (Fax)





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