[Histonet] Mice embryo cryocuts

Emily Sours talulahgosh <@t> gmail.com
Wed Aug 31 08:20:09 CDT 2011

You need to leave the embryos in sucrose waaay longer than 5 hours.  I would
leave them at least overnight, if not more than that.  You can probably take
them out of the blocks and resink them in sucrose, if you're desparate.
But next time, always sink (ie wash in sucrose) for at least overnight.  How
old are the mice you're sectioning?
Write back if that doesn't help!


A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron

On Wed, Aug 31, 2011 at 3:37 AM, Carmen Maria Garcia Pascual <
Carmen.M.Garcia <@t> uv.es> wrote:

> Good moorning everyone:
> I am Carmen Garcia from the University of Valencia. I am just starting
> my PhD. Now I have to obtain mice embryo cryocut and I am having some
> problems with the morphology... We removed the embryos and put them in
> sucrose 30% like 5 hours, then embebbed in OCT and put them at -80ºC,
> when we are going to cut them we let the in the cryostat like 2 hours
> and the cut them.
> Someone can give me any advise of what can we been doing wrong?
> Thank you so much.
> *******************************************************
> Carmen María García Pascual
> FIVI/INCLIVA/Facultad de Medicina
> Universidad de Valencia
> Departamento de P.O.G (Laboratorios)
> 96.386.40.48
> Avd. Blasco Ibañez 17
> 46010, Valencia
> *******************************************************
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