[Histonet] Mice embryo cryocuts

Carmen Maria Garcia Pascual Carmen.M.Garcia <@t> uv.es
Wed Aug 31 02:37:57 CDT 2011

Good moorning everyone:

I am Carmen Garcia from the University of Valencia. I am just starting 
my PhD. Now I have to obtain mice embryo cryocut and I am having some 
problems with the morphology... We removed the embryos and put them in 
sucrose 30% like 5 hours, then embebbed in OCT and put them at -80ºC, 
when we are going to cut them we let the in the cryostat like 2 hours 
and the cut them.
Someone can give me any advise of what can we been doing wrong?
Thank you so much.

Carmen María García Pascual
FIVI/INCLIVA/Facultad de Medicina
Universidad de Valencia
Departamento de P.O.G (Laboratorios)
Avd. Blasco Ibañez 17
46010, Valencia

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