[Histonet] Re: Histonet Digest, Vol 93, Issue 24

[Sherwood, Margaret]

I would like to give input on this problem.  We had a similar situation.  The
investigators couldn't flush the fecal material before fixing-destroyed the
treated area of interest.  After fixing, you couldn't flush the material out.
We ended up manually "picking out the material" before embedding (messy and not
fun!).  (P.S.  Food was withheld from the rats before euthanization).

If anyone has a suggestion, we would greatly appreciate hearing about it.

Peggy 

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea
L - (algranth)
Sent: Thursday, August 18, 2011 3:13 PM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Histonet Digest, Vol 93, Issue 24

Has she tried opening the colons and allowing them to fix and then gently
flushing the fecal matter out before processing?

Andi

On Aug 18, 2011, at 10:34 AM, Jennifer Sipes wrote:

> Hi everyone!
>  
> First let me thank EVERYONE for the huge response on the H&E stain on frozen
tissue!!! My slides turned out great!
>  
> Now, I've got a question that I'm thinking will stump you as much as it's
stumped a co-worker and I!  She is doing a study on the mucus membrane in the
intestinal tract(cecum and colon mainly) of the mouse.  She tried flushing the
fecal matter out of the colon and cecum but lost the membrane.  She decided to
leave the fecal matter in, however now when I section it, it tears up my blade
and gives sections that are not all that great.  I was wondering if anyone knows
how to acheive great sections while keeping the membrane?  
>  
> Thank you so much in advance for any and all help you can give us!
>  
> Regards,
>  
> Jen
>  
> Jennifer K. Sipes, ALAT 
> Sr. Laboratory Technician 
> Johns Hopkins University 
> Ross 933 
> 720 Rutland Avenue 
> Baltimore, MD  21205 
> phone:     410-614-0131 
> fax:         410-955-9677 
> cell:         443-631-6361 
> e-mail:  jsipes1 <@t> jhmi.edu 
> 
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> 



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