[Histonet] Re: Histonet Digest, Vol 93, Issue 24

[Grantham, Andrea L - (algranth)]

Has she tried opening the colons and allowing them to fix and then gently flushing the fecal matter out before processing?

Andi

On Aug 18, 2011, at 10:34 AM, Jennifer Sipes wrote:

> Hi everyone!
>  
> First let me thank EVERYONE for the huge response on the H&E stain on frozen tissue!!! My slides turned out great!
>  
> Now, I've got a question that I'm thinking will stump you as much as it's stumped a co-worker and I!  She is doing a study on the mucus membrane in the intestinal tract(cecum and colon mainly) of the mouse.  She tried flushing the fecal matter out of the colon and cecum but lost the membrane.  She decided to leave the fecal matter in, however now when I section it, it tears up my blade and gives sections that are not all that great.  I was wondering if anyone knows how to acheive great sections while keeping the membrane?  
>  
> Thank you so much in advance for any and all help you can give us!
>  
> Regards,
>  
> Jen
>  
> Jennifer K. Sipes, ALAT 
> Sr. Laboratory Technician 
> Johns Hopkins University 
> Ross 933 
> 720 Rutland Avenue 
> Baltimore, MD  21205 
> phone:     410-614-0131 
> fax:         410-955-9677 
> cell:         443-631-6361 
> e-mail:  jsipes1 <@t> jhmi.edu 
> 
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