[Histonet] Re: Histonet Digest, Vol 93, Issue 24
ttruscot <@t> vetmed.wsu.edu
Thu Aug 18 17:41:21 CDT 2011
Or, has you tried gently infusing the intestinal lumen with formalin from a syringe and tying or clamping both ends, then allow to fix in a jar of formalin, then trim open lengthways, then trim C-shaped cross-sections, then remove fecal material before placing in cassette. Tom T
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth)
Sent: Thursday, August 18, 2011 12:13 PM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Histonet Digest, Vol 93, Issue 24
Has she tried opening the colons and allowing them to fix and then gently flushing the fecal matter out before processing?
On Aug 18, 2011, at 10:34 AM, Jennifer Sipes wrote:
> Hi everyone!
> First let me thank EVERYONE for the huge response on the H&E stain on frozen tissue!!! My slides turned out great!
> Now, I've got a question that I'm thinking will stump you as much as it's stumped a co-worker and I! She is doing a study on the mucus membrane in the intestinal tract(cecum and colon mainly) of the mouse. She tried flushing the fecal matter out of the colon and cecum but lost the membrane. She decided to leave the fecal matter in, however now when I section it, it tears up my blade and gives sections that are not all that great. I was wondering if anyone knows how to acheive great sections while keeping the membrane?
> Thank you so much in advance for any and all help you can give us!
> Jennifer K. Sipes, ALAT
> Sr. Laboratory Technician
> Johns Hopkins University
> Ross 933
> 720 Rutland Avenue
> Baltimore, MD 21205
> phone: 410-614-0131
> fax: 410-955-9677
> cell: 443-631-6361
> e-mail: jsipes1 <@t> jhmi.edu
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