[Histonet] Details about Masson Trichrome Stain !

[Weems, Joyce]

http://stainsfile.info/StainsFile/dyes/26905.htm

This says 66...



Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of ? ?
Sent: Tuesday, August 16, 2011 13:14
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Details about Masson Trichrome Stain !

Details about Masson Trichrome Stain !
 
Biebrich Scarlet:
Biebrich scarlet 2.7 gm
Acid fuchsin 0.3 gm
Distilled water 300.0 ml
Glacial acetic acid 3.0 ml
 
I am not sure what the Biebrich Scarlet here is ? Acid Red 66 or acid Red 73 ?
 
Acid Red 73 http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=49823|FLUKA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
 
Acid Red 66 http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=B6008|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
 
What is the main difference between these two? 
May I choose 66, 73 or anyone?
 
Thanks !
 
Wang Lian, MD
Medical School of Nanjing University
Hankou Road 22#
Nanjing, Jiangsu Province, China
e-mail: wanglianebox <@t> yahoo.com.cn

 
 
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