[Histonet] IHC on Frozen Tissue

[Esparza, Sandra]

I leave out the cell conditioning step as well.  

Sandra
Sandra Esparza HT(ASCP)QIHC
Lead Technologist
Dell Children's Medical Center of Central Texas
512-324-0000  x87061
sesparza <@t> seton.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila
Fonner
Sent: Tuesday, August 16, 2011 6:44 AM
To: 'Yahoo'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC on Frozen Tissue

Does anyone else leave out the cell conditioning step?


-----Original Message-----
From: Yahoo [mailto:kim.tournear <@t> yahoo.com] 
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue

I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2. 

Sent from the iPhone of Kim Tournear

On Aug 16, 2011, at 6:03 AM, "Sheila Fonner" <sfonner <@t> labpath.com>
wrote:

> Thank you.
> 
> 
> -----Original Message-----
> From: Mierow, Brett T. [mailto:Brett.Mierow <@t> essentiahealth.org] 
> Sent: Tuesday, August 16, 2011 7:02 AM
> To: Sheila Fonner
> Subject: RE: [Histonet] IHC on Frozen Tissue
> 
> You are correct, leave out the depar. step. 
> 
> 
> Brett Mierow, HT (ASCP)
> Histology specialist
> Essentia Health
> SMDC Laboratory
> 407 East 3rd Street, Duluth, MN 54880 
> (218) 786-4510 | brett.mierow <@t> essentiahealth.org
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila
> Fonner
> Sent: Tuesday, August 16, 2011 5:41 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC on Frozen Tissue
> 
> Good morning everyone,
> 
> 
> 
> I was wondering if anyone could help me with a protocol change for the
> Ventana Ultra.  I have a pathologist that is requesting an HSVI,
HSVll,
> and Zoster Virus on frozen tissue that was received for IF studies. I
> know it's not Monday, but it is early, and I was hoping for some
> information about how to change the protocol for frozen vs. paraffin
> tissue.  Do I just need to delete the depar. step and leave everything
> else the same?  I appreciate any help you could give.
> 
> 
> 
> Thanks,
> 
> Sheila
> 
> 
> 
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