[Histonet] IHC on Frozen Tissue
Sheila Fonner
sfonner <@t> labpath.com
Tue Aug 16 06:44:19 CDT 2011
Does anyone else leave out the cell conditioning step?
-----Original Message-----
From: Yahoo [mailto:kim.tournear <@t> yahoo.com]
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue
I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2.
Sent from the iPhone of Kim Tournear
On Aug 16, 2011, at 6:03 AM, "Sheila Fonner" <sfonner <@t> labpath.com> wrote:
> Thank you.
>
>
> -----Original Message-----
> From: Mierow, Brett T. [mailto:Brett.Mierow <@t> essentiahealth.org]
> Sent: Tuesday, August 16, 2011 7:02 AM
> To: Sheila Fonner
> Subject: RE: [Histonet] IHC on Frozen Tissue
>
> You are correct, leave out the depar. step.
>
>
> Brett Mierow, HT (ASCP)
> Histology specialist
> Essentia Health
> SMDC Laboratory
> 407 East 3rd Street, Duluth, MN 54880
> (218) 786-4510 | brett.mierow <@t> essentiahealth.org
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila
> Fonner
> Sent: Tuesday, August 16, 2011 5:41 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC on Frozen Tissue
>
> Good morning everyone,
>
>
>
> I was wondering if anyone could help me with a protocol change for the
> Ventana Ultra. I have a pathologist that is requesting an HSVI, HSVll,
> and Zoster Virus on frozen tissue that was received for IF studies. I
> know it's not Monday, but it is early, and I was hoping for some
> information about how to change the protocol for frozen vs. paraffin
> tissue. Do I just need to delete the depar. step and leave everything
> else the same? I appreciate any help you could give.
>
>
>
> Thanks,
>
> Sheila
>
>
>
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