[Histonet] IHC on Frozen Tissue

[Sheila Fonner]

Rich,

 

Isn't the cell conditioning (retrieval) mostly done for tissues that have
been in formalin?  If the tissue is fresh (frozen), it has not seen
formalin.  That is the reason I questioned the cell conditioning step.

 

Thank you for your help.

Sheila

 

 

 

From: Richard Yeo [mailto:ryeo <@t> wchosp.org] 
Sent: Tuesday, August 16, 2011 7:54 AM
To: Sheila Fonner
Subject: RE: [Histonet] IHC on Frozen Tissue

 

Sheila,

 

The cell conditioning step is for retrieval. It will depend on what antibody
you are going to run. If you use it for Depar. you will also use it
wiith-out.

 

 

Rich Y

 

  _____  

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Sheila Fonner
Sent: Tue 8/16/2011 7:44 AM
To: 'Yahoo'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC on Frozen Tissue

Does anyone else leave out the cell conditioning step?


-----Original Message-----
From: Yahoo [mailto:kim.tournear <@t> yahoo.com]
Sent: Tuesday, August 16, 2011 7:41 AM
To: Sheila Fonner
Subject: Re: [Histonet] IHC on Frozen Tissue

I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2.

Sent from the iPhone of Kim Tournear

On Aug 16, 2011, at 6:03 AM, "Sheila Fonner" <sfonner <@t> labpath.com> wrote:

> Thank you.
>
>
> -----Original Message-----
> From: Mierow, Brett T. [mailto:Brett.Mierow <@t> essentiahealth.org]
> Sent: Tuesday, August 16, 2011 7:02 AM
> To: Sheila Fonner
> Subject: RE: [Histonet] IHC on Frozen Tissue
>
> You are correct, leave out the depar. step.
>
>
> Brett Mierow, HT (ASCP)
> Histology specialist
> Essentia Health
> SMDC Laboratory
> 407 East 3rd Street, Duluth, MN 54880
> (218) 786-4510 | brett.mierow <@t> essentiahealth.org
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila
> Fonner
> Sent: Tuesday, August 16, 2011 5:41 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC on Frozen Tissue
>
> Good morning everyone,
>
>
>
> I was wondering if anyone could help me with a protocol change for the
> Ventana Ultra.  I have a pathologist that is requesting an HSVI, HSVll,
> and Zoster Virus on frozen tissue that was received for IF studies. I
> know it's not Monday, but it is early, and I was hoping for some
> information about how to change the protocol for frozen vs. paraffin
> tissue.  Do I just need to delete the depar. step and leave everything
> else the same?  I appreciate any help you could give.
>
>
>
> Thanks,
>
> Sheila
>
>
>
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