[Histonet] Decalcification of bone

Rene J Buesa rjbuesa <@t> yahoo.com
Thu Aug 11 10:56:34 CDT 2011

As a matter of fact any decalciying technique presented in any histotechnique book works. The differences reside in the time it will take and the decalcifying method you use.
I will be very complicated to indicate you one, but a commercial productuct called "RDO" is one I used for hard bones and EDTA solution for bone marrow biopsies.
I this you will better of in consulting a techniques book and follow the instructions, especially those related to the "end point" of the decalcification.
René J.

--- On Thu, 8/11/11, Rachael Glebocki <Rachael.Glebocki <@t> nottingham.ac.uk> wrote:

From: Rachael Glebocki <Rachael.Glebocki <@t> nottingham.ac.uk>
Subject: [Histonet] Decalcification of bone
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, August 11, 2011, 4:10 AM

Dear Histonet users,

I was wondering if anyone has a operation procedure for bone decalcification that works. I am having no joy in decalcifying the bone and making a good slide from it.

Thank you for your time.

Rachael Glebocki
Teaching Technician
School of Veterinary Medicine & Science
University of Nottingham

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