[Histonet] Decalcification of bone

[Rene J Buesa]

As a matter of fact any decalciying technique presented in any histotechnique book works. The differences reside in the time it will take and the decalcifying method you use.
I will be very complicated to indicate you one, but a commercial productuct called "RDO" is one I used for hard bones and EDTA solution for bone marrow biopsies.
I this you will better of in consulting a techniques book and follow the instructions, especially those related to the "end point" of the decalcification.
René J.

--- On Thu, 8/11/11, Rachael Glebocki <Rachael.Glebocki <@t> nottingham.ac.uk> wrote:


From: Rachael Glebocki <Rachael.Glebocki <@t> nottingham.ac.uk>
Subject: [Histonet] Decalcification of bone
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, August 11, 2011, 4:10 AM


Dear Histonet users,

I was wondering if anyone has a operation procedure for bone decalcification that works. I am having no joy in decalcifying the bone and making a good slide from it.

Thank you for your time.

Rachael Glebocki
Teaching Technician
School of Veterinary Medicine & Science
University of Nottingham

This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it.   Please do not use, copy or disclose the information contained in this message or in any attachment.  Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.

This message has been checked for viruses but the contents of an attachment
may still contain software viruses which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK legislation._______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet