[Histonet] Mouse vs. Human cells

[Carl Postenka]

We've had succes identifying human cells in a mouse background using an antibody against human mitochondria (Neomarkers cat#MS-1372P). The primary is a monoclonal mouse anti-human, so we use a Dako ARK kit (#K3954) to eliminate the "mouse on mouse" background. HIER using citrate pH=6.0. H2O2 block. Primary diluted 1/100 (through ARK kit), for 20min @ R/T.
 

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Carl PostenkaLondon Regional Cancer Program


 > Date: Mon, 1 Aug 2011 12:55:54 -0500
> From: sdysart <@t> mirnarx.com
> To: Histonet <@t> lists.utsouthwestern.edu
> CC: 
> Subject: [Histonet] Mouse vs. Human cells
> 
> Hello Histo-hotties!
> 
> Question: We are working with xenograft tumors in mice.  The tumor cells
> are human in origin.  I am trying to come up with some kind of stain
> that will stain all the mouse cells and none of the human cells.  Just
> to be able to determine if any of the human tumor cells are in a normal
> looking say liver.  I was thinking maybe Ki-67?  It doesn't have to be
> any specific marker, just to be able to see a really blue mouse organ,
> and then if there are human cells of any type in the organ light up that
> one cell.
> 
> Thanks
> 
>  
> 
>  
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> 
> Histotechnologist
> 
> Mirna Therapeutics
> 
> 2150 Woodward Street
> 
> Suite 100
> 
> Austin, Texas  78744
> 
> (512)901-0900 ext. 6912
> 
>  
> 
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