[Histonet] Mouse vs. Human cells

[Kim Merriam]

Years ago, we used to distinguish human fibroblasts in mouse tissue with vimentin; which stains human cells quite nicely, but does not cross to mouse.
 
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

From: Carl Postenka <cpostenka <@t> hotmail.com>
To: sdysart <@t> mirnarx.com; histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, August 2, 2011 11:16 AM
Subject: RE: [Histonet] Mouse vs. Human cells


We've had succes identifying human cells in a mouse background using an antibody against human mitochondria (Neomarkers cat#MS-1372P). The primary is a monoclonal mouse anti-human, so we use a Dako ARK kit (#K3954) to eliminate the "mouse on mouse" background. HIER using citrate pH=6.0. H2O2 block. Primary diluted 1/100 (through ARK kit), for 20min @ R/T.


--------------------------------------
Carl PostenkaLondon Regional Cancer Program


> Date: Mon, 1 Aug 2011 12:55:54 -0500
> From: sdysart <@t> mirnarx.com
> To: Histonet <@t> lists.utsouthwestern.edu
> CC: 
> Subject: [Histonet] Mouse vs. Human cells
> 
> Hello Histo-hotties!
> 
> Question: We are working with xenograft tumors in mice.  The tumor cells
> are human in origin.  I am trying to come up with some kind of stain
> that will stain all the mouse cells and none of the human cells.  Just
> to be able to determine if any of the human tumor cells are in a normal
> looking say liver.  I was thinking maybe Ki-67?  It doesn't have to be
> any specific marker, just to be able to see a really blue mouse organ,
> and then if there are human cells of any type in the organ light up that
> one cell.
> 
> Thanks
> 
>  
> 
>  
> 
> Sarah Goebel-Dysart, BA, HT(ASCP)
> 
> Histotechnologist
> 
> Mirna Therapeutics
> 
> 2150 Woodward Street
> 
> Suite 100
> 
> Austin, Texas  78744
> 
> (512)901-0900 ext. 6912
> 
>  
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
                        _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet