[Histonet] Biopsy Run

[Rene J Buesa]

I am afraid your "decision" is wrong. "Empty nuclei" appears when the water between the section and the slide is not completely removed and "dissolves" the nuclear contents while drying and no staining after wards.
Check the sections draining for this artifact.
René J.

--- On Wed, 4/27/11, Marshall, Kimberly K <kkmarshall <@t> anthc.org> wrote:


From: Marshall, Kimberly K <kkmarshall <@t> anthc.org>
Subject: [Histonet] Biopsy Run
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, April 27, 2011, 8:21 AM


Morning and Happy Hump day Histo peeps

  Hope everyone is having a fun filled Lab Week, Here is Alaska we are
eating more than usual...

  Just need to ask one more question regarding my evil "nuclear
artifact".  We have decided it is a processing issue due to the type of
cassettes we use.  In cutting down the processing time, the reagents are
not able to get through the mesh on the cassettes.  I have ordered other
kinds but due to limited funds (cant get a new labeler) and even with
the special pens for writing on cassettes and slides the hand written
numbers are fading, I cant use them.  My next thought is to lengthen my
biopsy run.  Would you folks out there share your biopsy run times with
me?  I don't want to go back to having the over processing issues with
the biopsies that occur in the 12 hour, but maybe adding time would help
here.  

Thanks in advance and again hope everyone is having a great Lab week.

Kim
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