[Histonet] Biopsy Run

[Nicole Tatum]

I use the sponges and I keep them in a water so the formalin can penetrate
when placed in the processor. Make sure when you put the sponges in the
water that you mash them to get out the air bubbles. I use water just in
cause my little bowl spills. If it was in formalin I would have to break
down my whole grossing station and turn on hood. You can also use kim
wipes. Just cut you some squares and place tissue in middle then fold and
put in cassette.

Hope this helps.

Nicole







 And one more thing.... Soak the sponges in formalin... j
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yahoo
> Sent: Wednesday, April 27, 2011 08:56
> To: Marshall, Kimberly K
> Cc: Histonet
> Subject: Re: [Histonet] Biopsy Run
>
> Mesh cassettes are known to hold air pockets in them, so extending your
> times would only over process your tissue.
>
> I would suggest you use sponges, filter paper, or bx bags and use the
> cassettes you use for your larger specimens until you can get a different
> cassette.
>
> We found in our lab (we didn't have a printer) that the ink will fade if
> it's not dry enough before blocks are put in formalin container. Same on
> the slides.
>
> Hope this helps.
>
> Sent from the iPhone of Kim Tournear
>
> On Apr 27, 2011, at 7:21 AM, "Marshall, Kimberly K" <kkmarshall <@t> anthc.org>
> wrote:
>
>> Morning and Happy Hump day Histo peeps
>>
>>  Hope everyone is having a fun filled Lab Week, Here is Alaska we are
>> eating more than usual...
>>
>>  Just need to ask one more question regarding my evil "nuclear
>> artifact".  We have decided it is a processing issue due to the type
>> of cassettes we use.  In cutting down the processing time, the
>> reagents are not able to get through the mesh on the cassettes.  I
>> have ordered other kinds but due to limited funds (cant get a new
>> labeler) and even with the special pens for writing on cassettes and
>> slides the hand written numbers are fading, I cant use them.  My next
>> thought is to lengthen my biopsy run.  Would you folks out there share
>> your biopsy run times with me?  I don't want to go back to having the
>> over processing issues with the biopsies that occur in the 12 hour,
>> but maybe adding time would help here.
>>
>> Thanks in advance and again hope everyone is having a great Lab week.
>>
>> Kim
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