[Histonet] RE: Biopsy Run

[Tony Henwood]

Increase the fixation time. If they are small biopsies (otherwise why would you be putting them in mesh cassettes), you could easily reduce the processing time to 30 minutes (eg 4 alcohols, 2-3 xylenes, 2-3 waxes)

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K
Sent: Wednesday, 27 April 2011 10:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biopsy Run

Morning and Happy Hump day Histo peeps
 
  Hope everyone is having a fun filled Lab Week, Here is Alaska we are eating more than usual...
 
  Just need to ask one more question regarding my evil "nuclear artifact".  We have decided it is a processing issue due to the type of cassettes we use.  In cutting down the processing time, the reagents are not able to get through the mesh on the cassettes.  I have ordered other kinds but due to limited funds (cant get a new labeler) and even with the special pens for writing on cassettes and slides the hand written numbers are fading, I cant use them.  My next thought is to lengthen my biopsy run.  Would you folks out there share your biopsy run times with me?  I don't want to go back to having the over processing issues with the biopsies that occur in the 12 hour, but maybe adding time would help here.  
 
 Thanks in advance and again hope everyone is having a great Lab week.
 
Kim
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