[Histonet] How to keep paraffin sections from sticking to blade/blade holder.

[Eric Eades]

Hi Bret,

If you grab the first section as it comes off the blade with a pair of
forceps, then your ribbon never needs to touch the blade holder at all.
Just don't use your fingers!

Good luck,
-Eric

On Thu, Apr 21, 2011 at 12:07 PM, Clough, Bret
<Clough <@t> medicine.tamhsc.edu>wrote:

> While sectioning paraffin embedded tissue on a rotary  microtome I noticed
> the sections sticking to the blade holder and bunching up. What do I need to
> do so that my section come off the blade in a ribbon? Please note that this
> is my first time in sectioning tissue and this is a learning exercise  for
> me.
>
> Thanks in advance for your suggestions .
>
> Bret
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