[Histonet] endogenous peroxidases

[Emily Sours]

Hello

I know I can block endogenous peroxidases in paraffin sections before
antigen retrieval or after it, but which is better?
Does it make a difference?
Also, since our H2O2 is 30%, do I need to account for the water in it when
I'm diluting it in 100% MeOH?
(I was going to use a 0.3% H2O2 in MeOH wash for 30 minutes)
I'm not even sure how I would account for it, I just thought it might affect
the MeOH since it wouldn't be anhydrous anymore.

Emily
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